Act1 Antibody (WW-18): sc-100647

Act1 Antibody (WW-18) is a mouse monoclonal IgG2a kappa light chain antibody that detects Act1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Act1, also known as NFκB activator 1 or CIKS (connection to IKK and SAPK/JNK), plays a crucial role in the regulation of the NFκB and JNK signaling pathways, which are essential for immune and inflammatory responses, as well as cell survival under stress conditions. Act1 is primarily located in the cytoplasm, where Act1 associates with IKKγ, facilitating the activation of NFκB and JNK pathways. The proper functioning of Act1 is vital, as Act1 serves as a coordinator between these two critical stress-induced signaling pathways, ensuring that cells can effectively respond to various stimuli. Act1 (WW-18) antibody is available in a non-conjugated form, making Act1 (WW-18) antibody a versatile tool for researchers studying the intricate mechanisms of cellular signaling and the role of Act1 in health and disease.

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Act1 Antibody (WW-18) References:

1. Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity.  |  Karin, M. and Ben-Neriah, Y. 2000. Annu Rev Immunol. 18: 621-63. PMID: 10837071
2. Act1, an NF-kappa B-activating protein.  |  Li, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 10489-93. PMID: 10962024
3. CIKS, a connection to Ikappa B kinase and stress-activated protein kinase.  |  Leonardi, A., et al. 2000. Proc Natl Acad Sci U S A. 97: 10494-9. PMID: 10962033
4. Role of the adaptor protein CIKS in the activation of the IKK complex.  |  Mauro, C., et al. 2003. Biochem Biophys Res Commun. 309: 84-90. PMID: 12943667
5. Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells.  |  Huang, F., et al. 2007. J Immunol. 179: 6504-13. PMID: 17982039
6. Act1 modulates autoimmunity through its dual functions in CD40L/BAFF and IL-17 signaling.  |  Li, X. 2008. Cytokine. 41: 105-13. PMID: 18061473
7. Act1 is a negative regulator in T and B cells via direct inhibition of STAT3.  |  Zhang, CJ., et al. 2018. Nat Commun. 9: 2745. PMID: 30013031
8. IL-17A Promotes Psoriasis-Associated Keratinocyte Proliferation through ACT1-Dependent Activation of YAP-AREG Axis.  |  Yu, Z., et al. 2022. J Invest Dermatol. 142: 2343-2352. PMID: 35304250
9. Salidroside attenuates HALI via IL-17A-mediated ferroptosis of alveolar epithelial cells by regulating Act1-TRAF6-p38 MAPK pathway.  |  Guo, B., et al. 2022. Cell Commun Signal. 20: 183. PMID: 36411467
10. Act1 drives chemoresistance via regulation of antioxidant RNA metabolism and redox homeostasis.  |  Hong, L., et al. 2024. J Exp Med. 221: PMID: 38861022
11. Structure, regulation and function of NF-kappa B.  |  Siebenlist, U., et al. 1994. Annu Rev Cell Biol. 10: 405-55. PMID: 7888182
12. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases.  |  Barnes, PJ. and Karin, M. 1997. N Engl J Med. 336: 1066-71. PMID: 9091804

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Act1 Antibody (WW-18) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Act1 Antibody (WW-18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Act1 Antibody (WW-18) Specifications

Alternate Product Name: Act1

Product Clone Name: WW-18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 451-566

Epitope Target: Act1

Epitope Species: human

Product Reactivity/Detection: Act1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Act1 Antibody (WW-18): N/A

The preferred Act1 antibody is Act1 Antibody (D-11): sc-398161.