ACSS2 Antibody (A-9): sc-398559

ACSS2 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACSS2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACSS2 Antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACSS2, also known as acyl-CoA synthetase short-chain family member 2, is a 701 amino acid cytoplasmic protein that catalyzes the ATP-dependent activation of acetate to form acetyl-CoA, a key substrate for energy production and lipid biosynthesis. This enzymatic activity maintains energy homeostasis and supports various metabolic pathways, particularly in tissues with high energy demands such as the liver and kidney, where ACSS2 expression is most abundant. ACSS2 is regulated by unsaturated fatty acids and sterol regulatory element-binding proteins (SREBPs), highlighting its importance in lipid metabolism and cellular signaling. Human ACSS2 exists as two alternatively spliced isoforms and shares 93% sequence identity with its mouse counterpart, indicating a conserved functional role across species.

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ACSS2 Antibody (A-9) References:

1. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins.  |  Luong, A., et al. 2000. J Biol Chem. 275: 26458-66. PMID: 10843999
2. Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2.  |  Schwer, B., et al. 2006. Proc Natl Acad Sci U S A. 103: 10224-10229. PMID: 16788062
3. Phosphoproteome of resting human platelets.  |  Zahedi, RP., et al. 2008. J Proteome Res. 7: 526-34. PMID: 18088087
4. Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations.  |  Lu, Y., et al. 2008. J Lipid Res. 49: 2582-9. PMID: 18660489
5. Gene networks driving bovine milk fat synthesis during the lactation cycle.  |  Bionaz, M. and Loor, JJ. 2008. BMC Genomics. 9: 366. PMID: 18671863
6. Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy.  |  Li, X., et al. 2017. Mol Cell. 66: 684-697.e9. PMID: 28552616
7. Targeting ACSS2 with a Transition-State Mimetic Inhibits Triple-Negative Breast Cancer Growth.  |  Miller, KD., et al. 2021. Cancer Res. 81: 1252-1264. PMID: 33414169
8. ACSS2 gene variants determine kidney disease risk by controlling de novo lipogenesis in kidney tubules.  |  Mukhi, D., et al. 2023. J Clin Invest. 134: PMID: 38051585
9. Hypoxia upregulating ACSS2 enhances lipid metabolism reprogramming through HMGCS1 mediated PI3K/AKT/mTOR pathway to promote the progression of pancreatic neuroendocrine neoplasms.  |  Gu, D., et al. 2024. J Transl Med. 22: 93. PMID: 38263056
10. ACSS2 controls PPARγ activity homeostasis to potentiate adipose-tissue plasticity.  |  Chen, N., et al. 2024. Cell Death Differ. 31: 479-496. PMID: 38332049
11. Inhibition of ACSS2-mediated histone crotonylation alleviates kidney fibrosis via IL-1β-dependent macrophage activation and tubular cell senescence.  |  Li, L., et al. 2024. Nat Commun. 15: 3200. PMID: 38615014
12. ACSS2 acts as a lactyl-CoA synthetase and couples KAT2A to function as a lactyltransferase for histone lactylation and tumor immune evasion.  |  Zhu, R., et al. 2025. Cell Metab. 37: 361-376.e7. PMID: 39561764

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACSS2 Antibody (A-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-ACSS2 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACSS2 Antibody (A-9) Specifications

Alternate Product Name: ACSS2

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-160

Epitope Target: ACSS2

Epitope Species: human

Product Reactivity/Detection: ACSS2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for ACSS2 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin