ACSL3 Antibody (H-9): sc-166374

ACSL3 Antibody (H-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACSL3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ACSL3 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACSL3 protein, a member of the acyl-CoA synthetase family, plays a crucial role in lipid metabolism by catalyzing the conversion of long-chain fatty acids into acyl-CoA, which is essential for the synthesis of cellular lipids and the β-oxidation pathway. This enzymatic activity is vital for energy production and the formation of key lipid molecules such as triacylglycerols, phospholipids, and cholesteryl esters. ACSL3 is particularly important in tissues with high metabolic demands, such as the brain and heart, where ACSL3 preferentially utilizes fatty acids like laurate, myristate, arachidonate, and eicosapentaenoate. ACSL3 expression in various tissues, including the placenta, prostate, skeletal muscle, testis, and thymus, highlights diverse physiological roles, and ACSL3 regulation is critical for maintaining lipid homeostasis and overall cellular function.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ACSL3 Antibody (H-9) References:

1. Regulation by adrenocorticotropic hormone and arachidonate of the expression of acyl-CoA synthetase 4, an arachidonate-preferring enzyme expressed in steroidogenic tissues.  |  Cho, YY., et al. 2000. Biochem Biophys Res Commun. 274: 741-5. PMID: 10924347
2. Acyl-CoAs are functionally channeled in liver: potential role of acyl-CoA synthetase.  |  Muoio, DM., et al. 2000. Am J Physiol Endocrinol Metab. 279: E1366-73. PMID: 11093925
3. Abnormal uterus with polycysts, accumulation of uterine prostaglandins, and reduced fertility in mice heterozygous for acyl-CoA synthetase 4 deficiency.  |  Cho, YY., et al. 2001. Biochem Biophys Res Commun. 284: 993-7. PMID: 11409893
4. Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene.  |  Minekura, H., et al. 2001. Biochem Biophys Res Commun. 286: 80-6. PMID: 11485311
5. Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene.  |  Minekura, H., et al. 2001. Gene. 278: 185-92. PMID: 11707336
6. Do long-chain acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways?  |  Coleman, RA., et al. 2002. J Nutr. 132: 2123-6. PMID: 12163649
7. Baicalein ameliorates cerebral ischemia-reperfusion injury by inhibiting ferroptosis via regulating GPX4/ACSL4/ACSL3 axis.  |  Li, M., et al. 2022. Chem Biol Interact. 366: 110137. PMID: 36055377
8. ACSL3 regulates lipid droplet biogenesis and ferroptosis sensitivity in clear cell renal cell carcinoma.  |  Klasson, TD., et al. 2022. Cancer Metab. 10: 14. PMID: 36192773
9. ACSL3 and ACSL4, Distinct Roles in Ferroptosis and Cancers.  |  Yang, Y., et al. 2022. Cancers (Basel). 14: PMID: 36497375
10. ANKRD1 aggravates renal ischaemia‒reperfusion injury via promoting TRIM25-mediated ubiquitination of ACSL3.  |  Han, S., et al. 2024. Clin Transl Med. 14: e70024. PMID: 39285846
11. Molecular characterization and expression of rat acyl-CoA synthetase 3.  |  Fujino, T., et al. 1996. J Biol Chem. 271: 16748-52. PMID: 8663269
12. Alternative translation initiation generates acyl-CoA synthetase 3 isoforms with heterogeneous amino termini.  |  Fujino, T., et al. 1997. J Biochem. 122: 212-6. PMID: 9276691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACSL3 Antibody (H-9) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-ACSL3 Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACSL3 Antibody (H-9) Specifications

Alternate Product Name: ACSL3

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 645-672

Epitope Target: ACSL3

Epitope Species: human

Product Reactivity/Detection: ACSL3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for ACSL3 Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin