ACOT8 Antibody (C-3): sc-7343
- ACOT8 Antibody (C-3) is a mouse monoclonal IgG1 κ ACOT8 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-320 representing full length ACOT8 of human origin
- ACOT8 Antibody (C-3) is recommended for detection of ACOT8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ACOT8 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACOT8 Antibody (C-3) for WB applications. These reagents are now offered in bundles with ACOT8 Antibody (C-3) (see ordering information below).
ACOT8 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the ACOT8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACOT8 monoclonal antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACOT8, also known as Acyl-CoA thioesterase 8 or TEII p35, plays a crucial role in lipid metabolism by hydrolyzing acyl-CoA thioesters, which are important intermediates in fatty acid metabolism. This enzymatic activity is vital for maintaining cellular lipid homeostasis and regulating energy production, particularly in response to metabolic stress. Furthermore, ACOT8 has been shown to interact with the HIV protein Nef, a key factor in HIV pathogenesis that enhances viral replication and infectivity. The interaction between ACOT8 and Nef is associated with the downregulation of CD4, a critical receptor for HIV entry into host cells, indicating that ACOT8 may influence HIV pathogenesis through its role in modulating CD4 levels in infected cells. Notably, ACOT8 exhibits 42% identity to thioesterase II from Escherichia coli, yet does not share significant homology with previously cloned animal thioesterases, highlighting its unique evolutionary path and potential as a therapeutic target in HIV research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACOT8 Antibody (C-3) References:
- Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell. | Schwartz, O., et al. 1995. J Virol. 69: 4053-9. PMID: 7539505
- Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis. | Aiken, C. and Trono, D. 1995. J Virol. 69: 5048-56. PMID: 7541845
- The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes. | Spina, CA., et al. 1994. J Exp Med. 179: 115-23. PMID: 7903679
- The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. | Smith, S. 1994. FASEB J. 8: 1248-59. PMID: 8001737
- The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages. | Miller, MD., et al. 1994. J Exp Med. 179: 101-13. PMID: 8270859
- Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation. | Liu, LX., et al. 1997. J Biol Chem. 272: 13779-85. PMID: 9153233
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACOT8 Antibody (C-3) | sc-7343 | 200 µg/ml | $322.00 | |||
ACOT8 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-528177 | 200 µg Ab; 10 µg BP | $361.00 | |||
ACOT8 Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-520494 | 200 µg Ab, 40 µg BP | $361.00 | |||
ACOT8 Antibody (C-3): m-IgG1 BP-HRP Bundle | sc-542803 | 200 µg Ab; 20 µg BP | $361.00 | |||
ACOT8 Antibody (C-3) AC | sc-7343 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ACOT8 Antibody (C-3) HRP | sc-7343 HRP | 200 µg/ml | $322.00 | |||
ACOT8 Antibody (C-3) FITC | sc-7343 FITC | 200 µg/ml | $336.00 | |||
ACOT8 Antibody (C-3) PE | sc-7343 PE | 200 µg/ml | $349.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 488 | sc-7343 AF488 | 200 µg/ml | $364.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 546 | sc-7343 AF546 | 200 µg/ml | $364.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 594 | sc-7343 AF594 | 200 µg/ml | $364.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 647 | sc-7343 AF647 | 200 µg/ml | $364.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 680 | sc-7343 AF680 | 200 µg/ml | $364.00 | |||
ACOT8 Antibody (C-3) Alexa Fluor® 790 | sc-7343 AF790 | 200 µg/ml | $364.00 |