ACLP/AEBP1 Antibody (G-1): sc-271374. Western blot analysis of ACLP/AEBP1 expression in Hep G2 (A), Sol8 (C), A-10 (D), PC-12 (E) and L6 (F) whole cell lysates and human liver tissue extract (B). Detection reagent used: m-IgG1 BP-HRP: sc-525408.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACLP/AEBP1 Antibody (G-1): sc-271374. Western blot analysis of ACLP/AEBP1 expression in Hep G2 (A), Sol8 (C), A-10 (D), PC-12 (E) and L6 (F) whole cell lysates and human liver tissue extract (B). Detection reagent used: m-IgG1 BP-HRP: sc-525408.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
ACLP/AEBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACLP/AEBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACLP/AEBP1 Antibody (G-1): sc-271374