ACK Antibody (A-11): sc-28336

ACK Antibody (A-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ACK antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACK protein, also known as TNK2 or Activated Cdc42-Associated Kinase 1, plays a crucial role in cellular signaling pathways by acting as a tyrosine kinase that specifically interacts with the GTP-bound form of Cdc42Hs, a member of the Ras-related Rho subfamily of GTP-binding proteins. This interaction inhibits the GTPase activity of Cdc42Hs, thereby promoting its active state and influencing cytoskeletal organization and cellular morphology. A unique sequence of 47 amino acids located C-terminal to the SH3 domain of ACK is essential for this binding, highlighting ACK′s structural significance in maintaining the active form of Rho family GTPases. By sustaining the GTP-bound state of Cdc42Hs, ACK may play a pivotal role in various cellular processes, including cell migration, proliferation, and differentiation, making ACK an important target for research in cancer and other diseases where these pathways are dysregulated.

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ACK Antibody (A-11) References:

1. Structure of the small G protein Cdc42 bound to the GTPase-binding domain of ACK.  |  Mott, HR., et al. 1999. Nature. 399: 384-8. PMID: 10360579
2. The CDC42-specific inhibitor derived from ACK-1 blocks v-Ha-Ras-induced transformation.  |  Nur-E-Kamal, MS., et al. 1999. Oncogene. 18: 7787-93. PMID: 10618719
3. The tyrosine kinase ACK1 associates with clathrin-coated vesicles through a binding motif shared by arrestin and other adaptors.  |  Teo, M., et al. 2001. J Biol Chem. 276: 18392-8. PMID: 11278436
4. Cdc42-dependent nuclear translocation of non-receptor tyrosine kinase, ACK.  |  Ahmed, I., et al. 2004. Biochem Biophys Res Commun. 314: 571-9. PMID: 14733946
5. Ras-related GTPases and the cytoskeleton.  |  Hall, A. 1992. Mol Biol Cell. 3: 475-9. PMID: 1611153
6. Diversity and versatility of GTPase activating proteins for the p21rho subfamily of ras G proteins detected by a novel overlay assay.  |  Manser, E., et al. 1992. J Biol Chem. 267: 16025-8. PMID: 1644791
7. Development of cell polarity in budding yeast.  |  Drubin, DG. 1991. Cell. 65: 1093-6. PMID: 1905977
8. Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region gene.  |  Hall, C., et al. 1990. J Mol Biol. 211: 11-6. PMID: 2299665
9. Activated Cdc42-associated kinase 1 (ACK1) binds the sterile α motif (SAM) domain of the adaptor SLP-76 and phosphorylates proximal tyrosines.  |  Thaker, YR., et al. 2017. J Biol Chem. 292: 6281-6290. PMID: 28188290
10. Small Molecules Targeting Activated Cdc42-Associated Kinase 1 (ACK1/TNK2) for the Treatment of Cancers.  |  Wang, A., et al. 2021. J Med Chem. 64: 16328-16348. PMID: 34735773
11. Hepatocyte Growth Factor-Dependent Antiviral Activity of Activated cdc42-Associated Kinase 1 Against Hepatitis B Virus.  |  Lee, HW., et al. 2021. Front Microbiol. 12: 800935. PMID: 35003030
12. A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42.  |  Manser, E., et al. 1993. Nature. 363: 364-7. PMID: 8497321

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACK Antibody (A-11) has 40 citations in a variety of scientific publications.

Primary antibodies like Anti-ACK Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACK Antibody (A-11) Specifications

Alternate Product Name: Tyrosine Kinase Non Receptor 2

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 920-1036

Epitope Target: ACK

Epitope Species: human

Product Reactivity/Detection: ACK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 40 citations in various scientific publications

Available Conjugates/Forms for ACK Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin