ACK Antibody (A-11) recommended for detection of ACK of mouse, rat and human origin by WB, IP, IF and ELISA

ACK Antibody (A-11): sc-28336

ACK Antibody (A-11) is rated 4.9 out of 5 by 11.
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Datasheets

    • Anti-ACK Antibody (A-11) is a mouse monoclonal IgG2b (kappa light chain) ACK antibody provided at 200 µg/ml
    • raised against amino acids 920-1036 mapping at the C-terminus of ACK of human origin
    • Anti-ACK Antibody (A-11) is recommended for detection of ACK of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-ACK Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ACK (A-11): sc-28336.

What is the recommended secondary for this primary?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11

Does this antibody have a blocking peptide associated with it?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, unfortunately it is not possible to offer a blocking peptide for it.
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 5 out of 5 by from good product we did get good result from western blot experiment.
Date published: 2018-08-22
Rated 4 out of 5 by from Signal is strong 1:1000 in hela whole cell lysis for WB. Not that clean background.
Date published: 2017-07-28
Rated 5 out of 5 by from Western blot assay The antibody diluted in 5% milk in TBS-T. Secondary use anti-mouse HRP- linked antibodies were bound at 1/5000, I obtained specific band.
Date published: 2017-07-22
Rated 5 out of 5 by from specific band We observed specific band of ACK and low background in WB. Dilution of primary antibody is 1: 500.
Date published: 2017-06-03
Rated 5 out of 5 by from positive result Worked well for western with prostate tissue extracts,positive result.
Date published: 2017-05-25
Rated 5 out of 5 by from Good for IP western blot I used this antibody for my IP WB on human samples, and it gave me strong band
Date published: 2017-03-30
Rated 5 out of 5 by from Great wb !! Great antibody for western blot applications; strong signal
Date published: 2017-01-13
Rated 5 out of 5 by from Good for Western blot Great for mouse, rat, and human detection. The antibody has extensive Western blot data and several citations.
Date published: 2016-12-20
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