Acid Ceramidase Antibody (F-9): sc-518165
- Acid Ceramidase Antibody (F-9) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 292-316 of Acid Ceramidase β of human origin
- recommended for detection of Acid Ceramidase β of human origin by WB, IP, IF and ELISA
- Anti-Acid Ceramidase Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Acid Ceramidase Antibody (F-9) for WB applications. This reagent is now offered in a bundle with Acid Ceramidase Antibody (F-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
Acid Ceramidase Antibody (F-9) is a mouse monoclonal IgM kappa antibody that detects Acid Ceramidase β of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Acid Ceramidase (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Acid ceramidase plays a crucial role in sphingolipid metabolism by catalyzing ceramide degradation, a process vital for maintaining cellular homeostasis. Acid ceramidase deficiency leads to ceramide accumulation, a hallmark of Farber disease, causing severe early-onset joint and neurological issues in affected individuals. Bioinformatic analyses have identified acid ceramidase as one of the top five genes associated with melanoma, highlighting acid ceramidase′s potential role in cancer biology. Acid ceramidase not only performs ceramide hydrolysis but also synthesizes ceramide under certain conditions, utilizing [14C]lauric acid and sphingosine as substrates. Environmental pH significantly influences the reaction pathway, with a pH optimum of 4.5 favoring hydrolysis and a pH of 5.5 promoting synthesis, underscoring acid ceramidase′s complex regulatory mechanisms and central role in lipid metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Acid Ceramidase Antibody (F-9) References:
- The emergence of acid ceramidase as a therapeutic target for acute myeloid leukemia. | Tan, SF., et al. 2017. Expert Opin Ther Targets. 21: 583-590. PMID: 28434262
- Molecular Targeting of Acid Ceramidase in Glioblastoma: A Review of Its Role, Potential Treatment, and Challenges. | Nguyen, HS., et al. 2018. Pharmaceutics. 10: PMID: 29642535
- Sphingolipids and acid ceramidase as therapeutic targets in cancer therapy. | Govindarajah, N., et al. 2019. Crit Rev Oncol Hematol. 138: 104-111. PMID: 31092365
- Acid Ceramidase Rescues Cystic Fibrosis Mice from Pulmonary Infections. | Becker, KA., et al. 2021. Infect Immun. 89: PMID: 33139382
- Acid ceramidase, a double-edged sword in cancer aggression: A minireview. | Vethakanraj, HS., et al. 2020. Curr Cancer Drug Targets.. PMID: 33357194
- The Acid Ceramidase Is a SARS-CoV-2 Host Factor. | Geiger, N., et al. 2022. Cells. 11: PMID: 36010608
- Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis. | Xiao, J., et al. 2022. Mol Med. 28: 106. PMID: 36068514
- Acid ceramidase gene therapy ameliorates pulmonary arterial hypertension with right heart dysfunction. | Katz, MG., et al. 2023. Respir Res. 24: 197. PMID: 37568148
- Acid Ceramidase Inhibitor LCL-805 Antagonizes Akt Signaling and Promotes Iron-Dependent Cell Death in Acute Myeloid Leukemia. | Ung, J., et al. 2023. Cancers (Basel). 15: PMID: 38136410
- Targeting acid ceramidase enhances antitumor immune response in colorectal cancer. | Vijayan, Y., et al. 2024. J Adv Res. 65: 73-87. PMID: 38142035
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Acid Ceramidase Antibody (F-9) | sc-518165 | 200 µg/ml | $322.00 | |||
Acid Ceramidase Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-550672 | 200 µg Ab; 40 µg BP | $361.00 | |||
Acid Ceramidase Antibody (F-9) AC | sc-518165 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Acid Ceramidase Antibody (F-9) HRP | sc-518165 HRP | 200 µg/ml | $322.00 | |||
Acid Ceramidase Antibody (F-9) FITC | sc-518165 FITC | 200 µg/ml | $336.00 | |||
Acid Ceramidase Antibody (F-9) PE | sc-518165 PE | 200 µg/ml | $349.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 488 | sc-518165 AF488 | 200 µg/ml | $364.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 546 | sc-518165 AF546 | 200 µg/ml | $364.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 594 | sc-518165 AF594 | 200 µg/ml | $364.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 647 | sc-518165 AF647 | 200 µg/ml | $364.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 680 | sc-518165 AF680 | 200 µg/ml | $364.00 | |||
Acid Ceramidase Antibody (F-9) Alexa Fluor® 790 | sc-518165 AF790 | 200 µg/ml | $364.00 |