Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 Antibody (8E4): sc-293282

AChRα9 Antibody (8E4) is a mouse monoclonal IgG2a kappa light chain antibody that detects AChRα9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-AChRα9 antibody (8E4) is available as a non-conjugated isotype antibody. AChRα9 (8E4) antibody recognizes a protein that plays a crucial role in the auditory system, particularly in cochlear hair cells, where AChRα9 is essential for mediating efferent synaptic transmission between cholinergic olivocochlear fibers and outer hair cells (OHCs). AChRα9 represents the only nicotinic acetylcholine receptor found in cochlear hair cells, with expression localized primarily in the basal regions of adult rat cochlear OHCs and in the apical regions of inner hair cells (IHCs). AChRα9 channel facilitates calcium ion influx, which is vital for the modulation of auditory signals and the protection of hair cells from damage. Understanding specific interactions and functions of AChRα9 provides insights into hearing mechanisms and potential therapeutic targets for auditory disorders.

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Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 Antibody (8E4) References:

1. Role of alpha9 nicotinic ACh receptor subunits in the development and function of cochlear efferent innervation.  |  Vetter, DE., et al. 1999. Neuron. 23: 93-103. PMID: 10402196
2. Vestibular hair cells of the chick express the nicotinic acetylcholine receptor subunit alpha 9.  |  Lustig, LR., et al. 1999. J Vestib Res. 9: 359-67. PMID: 10544374
3. High calcium permeability and calcium block of the alpha9 nicotinic acetylcholine receptor.  |  Katz, E., et al. 2000. Hear Res. 141: 117-28. PMID: 10713500
4. Chromosome location and characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) gene.  |  Lustig, LR. and Peng, H. 2002. Cytogenet Genome Res. 98: 154-9. PMID: 12697997
5. Expression of nicotinic acetylcholine receptor subunit alpha9 in type II vestibular hair cells of rats.  |  Kong, WJ., et al. 2006. Acta Pharmacol Sin. 27: 1509-14. PMID: 17049129
6. Reflex Modification Audiometry Reveals Dual Roles for Olivocochlear Neurotransmission.  |  Allen, PD. and Luebke, AE. 2017. Front Cell Neurosci. 11: 361. PMID: 29213229
7. Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells.  |  Elgoyhen, AB., et al. 1994. Cell. 79: 705-15. PMID: 7954834
8. Cell-specific expression of the alpha 9 n-ACh receptor subunit in auditory hair cells revealed by single-cell RT-PCR.  |  Glowatzki, E., et al. 1995. Proc Biol Sci. 262: 141-7. PMID: 8524909
9. Developmental expression of alpha 9 acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear.  |  Luo, L., et al. 1998. J Comp Neurol. 393: 320-31. PMID: 9548553
10. Differential expression of the alpha 9 nicotinic acetylcholine receptor subunit in neonatal and adult cochlear hair cells.  |  Simmons, DD. and Morley, BJ. 1998. Brain Res Mol Brain Res. 56: 287-92. PMID: 9602155
11. The alpha9 nicotinic acetylcholine receptor shares pharmacological properties with type A gamma-aminobutyric acid, glycine, and type 3 serotonin receptors.  |  Rothlin, CV., et al. 1999. Mol Pharmacol. 55: 248-54. PMID: 9927615