AChR alpha 9 Antibody (8E4) recommended for detection of AChRα9 of mouse, rat and human origin by WB, IP and ELISA

AChRα9 Antibody (8E4): sc-293282

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Datasheets

    • Anti-AChRα9 Antibody (8E4) is a mouse monoclonal IgG2a κ AChRα9 antibody, cited in 3 publications, provided at 100 µg/ml
    • raised against amino acids 139-221 of AChRα9 of human origin
    • recommended for detection of AChRα9 of mouse, rat and human origin by WB, IP and ELISA

I saw two clear bands in the WB picture showed on your website. And the isotype control is igG2a Kappa. My question is that the mAb is the full length of heavy chain and a light chain, or purified IgG2a kappa chain. Thank you.

Asked by: yu long
Thank you for your question. The Western blot data you are referring to on our website is not representative of the endogenous molecular weight for AChRα9. That is Western blot data in a human recombinant fusion protein. This is a complete IgG2a antibody. The light chain type is kappa.
Answered by: Technical Support
Date published: 2019-07-13

For Western Blot, is it recommended to use denatured or non-denatured conditions with AChRα9 (8E4): sc-293282 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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