ACD Antibody (A-8): sc-377318
- ACD Antibody (A-8) is a mouse monoclonal IgG1 κ ACD antibody provided at 200 µg/ml
- raised against amino acids 117-416 mapping at the C-terminus of ACD of mouse origin
- ACD Antibody (A-8) is recommended for detection of ACD of mouse and rat origin by WB, IP, IF and ELISA
- Anti-ACD Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACD Antibody (A-8) for WB applications. These reagents are now offered in bundles with ACD Antibody (A-8) (see ordering information below).
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ACD Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACD protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ACD antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACD, also known as adrenocortical dysplasia homolog, PIP1, PTOP, TPP1, or TINT1, consists of 544 amino acids and serves as a crucial component of the telosome/shelterin complex, which plays a vital role in maintaining and protecting telomeres. Located in the nucleus, ACD is essential for the proper assembly and stabilization of the telomere-associated complex, directly interacting with other key proteins such as protection of telomeres 1 (POT1) and telomeric repeat binding factor 1 (TRF1). These interactions are critical for regulating telomere length and elongation, as ACD mediates telomerase activity and facilitates telomerase access to DNA. The ability of ACD to control telomere dynamics is significant for cellular aging and organogenesis, highlighting its importance in genomic stability and cellular function. Additionally, alternative splicing of the ACD gene results in the expression of two isoforms, further diversifying its functional roles within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACD Antibody (A-8) References:
- PTOP interacts with POT1 and regulates its localization to telomeres. | Liu, D., et al. 2004. Nat Cell Biol. 6: 673-80. PMID: 15181449
- POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex. | Ye, JZ., et al. 2004. Genes Dev. 18: 1649-54. PMID: 15231715
- Shelterin: the protein complex that shapes and safeguards human telomeres. | de Lange, T. 2005. Genes Dev. 19: 2100-10. PMID: 16166375
- A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly. | O'Connor, MS., et al. 2006. Proc Natl Acad Sci U S A. 103: 11874-9. PMID: 16880378
- TPP1 is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase. | Xin, H., et al. 2007. Nature. 445: 559-62. PMID: 17237767
- The POT1-TPP1 telomere complex is a telomerase processivity factor. | Wang, F., et al. 2007. Nature. 445: 506-10. PMID: 17237768
- Telomerase unplugged. | Cristofari, G., et al. 2007. ACS Chem Biol. 2: 155-8. PMID: 17373762
- Telomere protection by mammalian Pot1 requires interaction with Tpp1. | Hockemeyer, D., et al. 2007. Nat Struct Mol Biol. 14: 754-61. PMID: 17632522
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACD Antibody (A-8) | sc-377318 | 200 µg/ml | $322.00 | |||
ACD Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-530005 | 200 µg Ab; 10 µg BP | $361.00 | |||
ACD Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-523278 | 200 µg Ab, 40 µg BP | $361.00 | |||
ACD Antibody (A-8) AC | sc-377318 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ACD Antibody (A-8) HRP | sc-377318 HRP | 200 µg/ml | $322.00 | |||
ACD Antibody (A-8) FITC | sc-377318 FITC | 200 µg/ml | $336.00 | |||
ACD Antibody (A-8) PE | sc-377318 PE | 200 µg/ml | $349.00 | |||
ACD Antibody (A-8) Alexa Fluor® 488 | sc-377318 AF488 | 200 µg/ml | $364.00 | |||
ACD Antibody (A-8) Alexa Fluor® 546 | sc-377318 AF546 | 200 µg/ml | $364.00 | |||
ACD Antibody (A-8) Alexa Fluor® 594 | sc-377318 AF594 | 200 µg/ml | $364.00 | |||
ACD Antibody (A-8) Alexa Fluor® 647 | sc-377318 AF647 | 200 µg/ml | $364.00 | |||
ACD Antibody (A-8) Alexa Fluor® 680 | sc-377318 AF680 | 200 µg/ml | $364.00 | |||
ACD Antibody (A-8) Alexa Fluor® 790 | sc-377318 AF790 | 200 µg/ml | $364.00 |