Ac-Histone H4 Antibody (E-5): sc-377520

Ac-Histone H4 Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ac-Histone H4 protein of broad species origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Ac-Histone H4 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ac-Histone H4 protein plays a crucial role in regulating gene expression through involvement in chromatin structure and dynamics. Specifically, acetylation of histone H4 at specific lysine residues, such as lysines 5 and 8, is essential for chromatin relaxation, allowing transcription factors and other regulatory proteins to access DNA. This post-translational modification is vital for processes such as DNA repair, replication, and transcriptional activation, highlighting Ac-Histone H4′s importance in maintaining cellular function and responding to environmental signals. The dynamic interplay between acetylated histones and chromatin remodeling complexes underscores Ac-Histone H4′s significance in epigenetic regulation, making Ac-Histone H4 (E-5) antibody an invaluable tool for researchers studying gene expression and chromatin biology.

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Ac-Histone H4 Antibody (E-5) References:

1. Curcumin, a potent anti-tumor reagent, is a novel histone deacetylase inhibitor regulating B-NHL cell line Raji proliferation.  |  Liu, HL., et al. 2005. Acta Pharmacol Sin. 26: 603-9. PMID: 15842781
2. The effect of smoking on the transcriptional regulation of lung inflammation in patients with chronic obstructive pulmonary disease.  |  Szulakowski, P., et al. 2006. Am J Respir Crit Care Med. 174: 41-50. PMID: 16574938
3. Single agent efficacy of the HDAC inhibitor DATS in preclinical models of glioblastoma.  |  Das, A., et al. 2018. Cancer Chemother Pharmacol. 82: 945-952. PMID: 30209569
4. Pracinostat (SB939), a histone deacetylase inhibitor, suppresses breast cancer metastasis and growth by inactivating the IL-6/STAT3 signalling pathways.  |  Chen, J., et al. 2020. Life Sci. 248: 117469. PMID: 32109485
5. DDT tolerance in diazinon-selected and DDT-selected strains of houseflies.  |  el Basheir el-S,. and Lord, KA. 1965. Chem Ind. 37: 1598-9. PMID: 5842781
6. Curcumin, a potent anti-tumor reagent, is a novel histone deacetylase inhibitor regulating B-NHL cell line Raji proliferation  |  Hong-li Liu, et al. 2005. APS. 603-609.
7. AID-dependent histone acetylation is detected in immunoglobulin S regions  |  Lilli Wang, et al. 2006. JEM. 203: 215-226.
8. Experimental in vivo and in vitro treatment with a new histone deacetylase inhibitor belinostat inhibits the growth of pancreatic cancer  |  Dimitriy Dovzhanskiy, et al. 2012. Springer Nature Link. 12: 226.
9. Pracinostat (SB939), a histone deacetylase inhibitor, suppresses breast cancer metastasis and growth by inactivating the IL-6/STAT3 signalling pathways  |  Jing Chen, et al. 2020. ELSEVIER. 248.
10. Acquired Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) Resistance of Human Colorectal Cancer Cells Is Linked to Histone Acetylation and Is Synergistically Ameliorated by Combination with HDAC Inhibitors  |  se Lim Kim, et al. 2024. Springer Nature Link. 669: 3305-3317.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ac-Histone H4 Antibody (E-5) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-Ac-Histone H4 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ac-Histone H4 Antibody (E-5) Specifications

Alternate Product Name: Acetyl Histone H4

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Histone H4

Epitope Species: human

Product Reactivity/Detection: Ser 1, Lys 5, Lys 8 and Lys 12 acetylated Histone H4

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for Ac-Histone H4 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Histone H4 antibody is Histone H4 Antibody (F-9): sc-25260.