Anti-Ac-Histone H4 Antibody (E-5) recommended for detection of Ser 1, Lys 5, Lys 8 and Lys 12 acetylated Histone H4 of broad species and origin by WB, IP, IF, IHC(P) and ELISA

Anti-Ac-Histone H4 Antibody (E-5): sc-377520

Anti-Ac-Histone H4 Antibody (E-5) is rated 4.9 out of 5 by 8.
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Datasheets

    • Anti-Ac-Histone H4 Antibody (E-5) is a mouse monoclonal IgG2a κ Ac-Histone H4 antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping to a short peptide containing acetylated Serine 1 and Lysine 5, 8 and 12 of Histone H4 of human origin
    • Ac-Histone H4 Antibody (E-5) is recommended for detection of Ser 1, Lys 5, Lys 8 and Lys 12 acetylated Histone H4 of broad species and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-Ac-Histone H4 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ac-Histone H4 (E-5): sc-377520.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ac-Histone H4 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Ac-Histone H4 Antibody (E-5) (see ordering information below).
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Is this antibody suitable for conjugation with an NHS Ester dye?

Asked by: sc j
Thank you for your question. This antibody is suitable for labeling with NHS ester dyes. For custom conjugations, please email [email protected] or contact our European support office for assistance. You may reach them toll-free by phone at 00800 4573 8000, by email at [email protected], or contact them by live chat on our website at www.scbt.com. Alternatively, you can contact one of our authorized distributors in region, which can be found in the following link: https://www.scbt.com/scbt/resources/distributors
Answered by: Blake J
Date published: 2021-07-12

What application is the blocking peptide sc-377520 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody It works very well for my Western blot with 1:200 duiltion
Date published: 2018-04-12
Rated 5 out of 5 by from very useful in WB I got this antibody(E-5) almost two weeks ago,I found it was very useful in my WB experiments in the ratio 1:750. In fact, I got perfect result only one chance.So excited!
Date published: 2017-12-19
Rated 5 out of 5 by from This antibody was confirmed to be useful! I used the WB method to detect Ac-Histone H4 in UMUC3 cell line. The antibody (1: 200) is easy to detect the expression of Ac-Histone H4. The background is very clean and the molecular weight is also accurate.
Date published: 2017-11-01
Rated 4 out of 5 by from good I used this antibody in the ratio 1:500 in hela cell line and I got a clear brand
Date published: 2017-08-08
Rated 5 out of 5 by from Great ab Strong bands in WB experiments in cells treated with histone deacetylase inhibitor.
Date published: 2017-03-10
Rated 5 out of 5 by from IF: Nice nuclear staining with NIH/3T3 IF: Nice nuclear staining with NIH/3T3. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in NIH/3T3 and Trichostatin A treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HepG2. -SCBT QC
Date published: 2014-03-28
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