ABHD5 Antibody (E-1): sc-376931

ABHD5 Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects ABHD5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ABHD5 Antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ABHD5, also known as CGI-58, NCIE2, or CDS, is a 349 amino acid protein characterized by an alpha/beta-hydrolase domain that plays a crucial role in lipid metabolism. ABHD5 is primarily localized to the surface of lipid droplets, where ABHD5 interacts with Perilipin, facilitating lipolysis—the breakdown of stored fats into free fatty acids, which are essential for energy production and cellular function. ABHD5′s importance in lipid metabolism is underscored by its association with Chanarin-Dorfman syndrome (CDS), a genetic disorder resulting from defects in the ABHD5 gene, leading to impaired long-chain fatty acid oxidation and a range of symptoms including congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss, and developmental delay. anti-ABHD5 antibody (E-1) effectively detects this critical protein, making ABHD5 (E-1) monoclonal antibody an invaluable tool for researchers studying lipid metabolism and related disorders.

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ABHD5 Antibody (E-1) References:

1. Mutations in CGI-58, the gene encoding a new protein of the esterase/lipase/thioesterase subfamily, in Chanarin-Dorfman syndrome.  |  Lefèvre, C., et al. 2001. Am J Hum Genet. 69: 1002-12. PMID: 11590543
2. Two new mutations of the ABHD5 gene in a new adult case of Chanarin Dorfman syndrome: an uncommon lipid storage disease.  |  Schleinitz, N., et al. 2005. Arch Dermatol. 141: 798-800. PMID: 15967942
3. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome.  |  Lass, A., et al. 2006. Cell Metab. 3: 309-19. PMID: 16679289
4. CGI-58 facilitates lipolysis on lipid droplets but is not involved in the vesiculation of lipid droplets caused by hormonal stimulation.  |  Yamaguchi, T., et al. 2007. J Lipid Res. 48: 1078-89. PMID: 17308334
5. A novel S115G mutation of CGI-58 in a Turkish patient with Dorfman-Chanarin syndrome.  |  Ben Selma, Z., et al. 2007. J Invest Dermatol. 127: 2273-6. PMID: 17495960
6. [Neutral lipid storage diseases and ATGL (adipose triglyceride lipase) and CGI-58/ABHD5 (alpha-beta hydrolase domain-containing 5) deficiency: myopathy, ichthyosis, but no obesity].  |  Fischer, J., et al. 2007. Med Sci (Paris). 23: 575-8. PMID: 17631826
7. ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production.  |  Vieyres, G., et al. 2016. PLoS Pathog. 12: e1005568. PMID: 27124600
8. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.  |  Miao, H., et al. 2016. Nat Commun. 7: 11716. PMID: 27189574
9. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2.  |  Peng, Y., et al. 2016. Autophagy. 12: 2167-2182. PMID: 27559856
10. Loss of ABHD5 promotes the aggressiveness of prostate cancer cells.  |  Chen, G., et al. 2017. Sci Rep. 7: 13021. PMID: 29026202
11. ABHD5 stimulates PNPLA1-mediated ω-O-acylceramide biosynthesis essential for a functional skin permeability barrier.  |  Kien, B., et al. 2018. J Lipid Res. 59: 2360-2367. PMID: 30361410
12. The ATGL lipase cooperates with ABHD5 to mobilize lipids for hepatitis C virus assembly.  |  Vieyres, G., et al. 2020. PLoS Pathog. 16: e1008554. PMID: 32542055

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ABHD5 Antibody (E-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ABHD5 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ABHD5 Antibody (E-1) Specifications

Alternate Product Name: ABHD5

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 179-226

Epitope Target: ABHD5

Epitope Species: human

Product Reactivity/Detection: ABHD5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ABHD5 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin