Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ABCF1 CRISPR/Cas9 KO Plasmid (h): sc-405684
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABCF1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ABCF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABCF1 Antibody (H-3): sc-377185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABCF1 CRISPR/Cas9 KO Plasmid (h) | sc-405684 | 20 µg | $397.00 |
Overview
ABCF1 (ATP-binding cassette sub-family F member 1) is an essential cytosolic ABC protein that lacks transmembrane domains and functions primarily in translation initiation and ribosome-associated processes. It interacts with components of the eIF2/eIF2B axis and other initiation factors, linking ATP-dependent regulation to protein synthesis control during cellular stress and innate immune signaling. ABCF1 has also been implicated in NF-κB–related inflammatory pathways and nucleic acid–triggered responses, connecting translational regulation with antiviral and cytokine programs. Dysregulated ABCF1 expression or activity has been associated with altered proliferation and immune phenotypes in cancer and inflammatory disease models, supporting its use in mechanistic studies of stress adaptation and host defense.
ABCF1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ABCF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ABCF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ABCF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ABCF1 protein expression.
This CRISPR knockout system enables efficient generation of ABCF1-deficient cell models for investigation of ABCF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ABCF1 exon(s) critical for ABCF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ABCF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ABCF1 CRISPR/Cas9 KO Plasmid (h) and ABCF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ABCF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ABCF1 HDR Plasmid (h) and ABCF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ABCF1 homology arms to support homology-directed repair at defined ABCF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.