Anti-ABCD3 Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) ABCD3 antibody provided at 200 µg/ml
raised against amino acids 280-475 of ABCD3 of human origin
ABCD3 Antibody (F-1) is recommended for detection of ABCD3 of human origin by WB, IP, IF and ELISA
Anti-ABCD3 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABCD3 (F-1): sc-514728.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ABCD3 Antibody (F-1) is a high quality monoclonal ABCD3 antibody (also designated ABCD3 antibody) suitable for the detection of the ABCD3 protein of human origin. ABCD3 Antibody (F-1) is available as both the non-conjugated anti-ABCD3 antibody form, as well as multiple conjugated forms of anti-ABCD3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The peroxisomal membrane contains several ATP-binding cassette (ABC) transporters, ABCD1-4 that are known to be present in the human peroxisome membrane. All four proteins are ABC half-transporters, which dimerize to form an active transporter. A mutation in the ABCD1 gene causes X-linked adreno-leukodystrophy (X-ALD), a peroxisomal disorder which affects lipid storage. ABCD2 in mouse is expressed at high levels in the brain and adrenal organs, which are adversely affected in X-ALD. The peroxisomal membrane comprises two quantitatively major proteins, PMP22 and ABCD3. ABCD3 is associated with irregularly shaped vesicles which may be defective peroxisomes or peroxisome precursors. ABCD1 localizes to peroxisomes. ABCB7 is a half-transporter involved in the transport of heme from the mitochondria to the cytosol.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For Western Blot, is it recommended to use denatured or non-denatured conditions with ABCD3 (F-1): sc-514728 monoclonal antibody?
Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 1 out of
Did not work for IFSThis antibody was tested for immunofluorescent staining of primary murine bone marrow-derived eosinophils. ABCD3 (F1) did not work for immunofluorescent staining in 4% paraformaldehyde-fixed/0.2%Triton X-100-permeabilized cells.
Date published: 2017-11-06
Rated 5 out of
Good for WB and IFIt works good for western blot and IF in HepG2 cells. the dilution is 1:500.
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