ABCA2 CRISPR/Cas9 KO Plasmid (m): sc-418931
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABCA2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ABCA2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABCA2 Antibody (9A2): sc-130575
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABCA2 CRISPR/Cas9 KO Plasmid (m) | sc-418931 | 20 µg | $397.00 |
Overview
Abca2 encodes ABCA2, an ATP-binding cassette transporter predominantly localized to endolysosomal membranes in mouse cells, where it contributes to intracellular lipid handling and vesicular trafficking. ABCA2 has been linked to regulation of cholesterol and sphingolipid homeostasis, impacting membrane composition and processes such as endocytosis, lysosome function, and myelin-associated lipid biology. Altered ABCA2 activity has been associated with neuronal and glial biology, neurodegeneration-relevant pathways, and multidrug resistance phenotypes in experimental systems. As a result, Abca2 is frequently studied in contexts connecting lipid transport to stress responses, proteostasis, and cell-type-specific vulnerability in the nervous system.
ABCA2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Abca2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Abca2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Abca2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ABCA2 protein expression.
This CRISPR knockout system enables efficient generation of Abca2-deficient cell models for investigation of ABCA2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Abca2 exon(s) critical for ABCA2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Abca2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ABCA2 CRISPR/Cas9 KO Plasmid (m) and ABCA2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Abca2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ABCA2 HDR Plasmid (m) and ABCA2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Abca2 homology arms to support homology-directed repair at defined Abca2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.