AAT Antibody (H-7): sc-166018

AAT Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects AAT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AAT antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AAT protein, also known as alpha-1-antitrypsin, plays a crucial role in protecting lung tissue from damage caused by neutrophil elastase, an enzyme that can lead to pulmonary emphysema when left unchecked. This 394 amino acid hepatic acute phase protein is primarily synthesized in the liver and is also expressed in macrophages, where AAT helps maintain the balance of proteolytic activity in the lungs. AAT is a highly polymorphic glycoprotein, and AAT variants exhibit distinct isoelectric-focusing patterns, which can be important for understanding individual susceptibility to lung diseases. The gene encoding AAT is located on human chromosome 14, adjacent to a related serine protease inhibitor gene that encodes corticosteroid-binding globulin. Notably, oxidation of the methionine 358 residue in AAT′s active site significantly reduces AAT′s ability to inhibit elastase, highlighting the importance of post-translational modifications in regulating function. A deficiency in AAT can lead to a 20-30 fold increased risk of developing early-onset pulmonary emphysema, making the study of AAT protein and its interactions vital for understanding respiratory health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AAT Antibody (H-7) References:

1. Hepatocellular ubiquitin expression in alpha1-antitrypsin deficiency.  |  Wu, SS., et al. 2001. Pediatr Dev Pathol. 4: 344-50. PMID: 11441335
2. Hypersensitive mousetraps, alpha1-antitrypsin deficiency and dementia.  |  Lomas, DA., et al. 2002. Biochem Soc Trans. 30: 89-92. PMID: 12023831
3. Liver disease associated with alpha1-antitrypsin deficiency.  |  Rosen, HR. 1998. Clin Liver Dis. 2: 175-85. PMID: 15560052
4. Ventilation inhomogeneity in alpha1-antitrypsin-deficient emphysema.  |  Fregonese, L., et al. 2006. Eur Respir J. 28: 323-9. PMID: 16571611
5. α1-antitrypsin production by proinflammatory and antiinflammatory macrophages and dendritic cells.  |  van 't Wout, EF., et al. 2012. Am J Respir Cell Mol Biol. 46: 607-13. PMID: 22162908
6. Serum α-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome.  |  Sarabhai, T., et al. 2017. PLoS One. 12: e0177450. PMID: 28493974
7. Liver disease in adults with α1-antitrypsin deficiency.  |  Mandorfer, M., et al. 2018. United European Gastroenterol J. 6: 710-718. PMID: 30083333
8. Fibrosis, biomarkers and liver biopsy in AAT deficiency and relation to liver Z protein polymer accumulation.  |  Suri, A., et al. 2024. Liver Int. 44: 3204-3213. PMID: 39263815
9. Pulmonary emphysema and alpha1-antitrypsin deficiency.  |  Jones, SH. 1974. Phys Ther. 54: 579-83. PMID: 4214194
10. Alpha-1-antitrypsin (AAT) deposits in gall bladder adenocarcinoma and liver in partial AAT deficiency (Pi SZ phenotype).  |  Callea, F., et al. 1982. Am J Clin Pathol. 78: 878-83. PMID: 6983292

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AAT Antibody (H-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AAT Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AAT Antibody (H-7) Specifications

Alternate Product Name: SERPINA1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 216-418

Epitope Target: AAT

Epitope Species: human

Product Reactivity/Detection: AAT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AAT Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred AAT antibody is AAT Antibody (B9): sc-59438.