A20/TNFAIP3 Antibody (8E8.38): sc-32797

A20 Antibody (8E8.38) is a mouse monoclonal IgG1 antibody that detects A20 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). A20 is a crucial Cys2/Cys2 zinc finger protein that is upregulated in response to various inflammatory stimuli, including tumor necrosis factor (TNF) and interleukin 1 (IL-1), and serves as a negative regulator of nuclear factor kappa B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays an essential role in the resolution of inflammatory responses, thereby preventing excessive cellular damage and promoting tissue homeostasis. A20′s structure is particularly significant, as A20 contains seven zinc finger domains in its C-terminal region, with only four being necessary for the in vitro inhibition of TNF-induced NFκB activation. This structural feature allows A20 to effectively interact with key signaling proteins such as TRAF2, TRAF6, and IkB kinase (IKK) γ, which are vital for modulating cell survival and apoptosis. Additionally, A20′s interaction with TXBP151, a novel A20-binding protein, suggests a mechanism through which A20 exerts anti-apoptotic effects. Given A20′s role in negative feedback regulation of signal transduction pathways, A20 and associated proteins hold promise as potential therapeutic targets for a range of inflammatory diseases.

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A20/TNFAIP3 Antibody (8E8.38) References:

1. A20 in inflammation and autoimmunity.  |  Catrysse, L., et al. 2014. Trends Immunol. 35: 22-31. PMID: 24246475
2. Haploinsufficiency of A20 (HA20): updates on the genetics, phenotype, pathogenesis and treatment.  |  Yu, MP., et al. 2020. World J Pediatr. 16: 575-584. PMID: 31587140
3. A20 and Cell Death-driven Inflammation.  |  Priem, D., et al. 2020. Trends Immunol. 41: 421-435. PMID: 32241683
4. The Ubiquitin System and A20: Implications in Health and Disease.  |  Mooney, EC. and Sahingur, SE. 2021. J Dent Res. 100: 10-20. PMID: 32853526
5. A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS.  |  Johann, L., et al. 2023. J Clin Invest. 133: PMID: 37856217
6. Protein citrullination and NET formation do not contribute to the pathology of A20/TNFAIP3 mutant mice.  |  Van Damme, KFA., et al. 2023. Sci Rep. 13: 17992. PMID: 37865713
7. A20 ameliorates Aspergillus fumigatus keratitis by promoting autophagy and inhibiting NF-κB signaling.  |  Han, F., et al. 2023. Int J Biol Macromol. 253: 127640. PMID: 37879579
8. ADAR1 protects pulmonary macrophages from sepsis-induced pyroptosis and lung injury through miR-21/A20 signaling.  |  Zhao, X., et al. 2024. Int J Biol Sci. 20: 464-485. PMID: 38169584
9. Inhibition of NLRP3 inflammasome activation by A20 through modulation of NEK7.  |  Yu, J., et al. 2024. Proc Natl Acad Sci U S A. 121: e2316551121. PMID: 38865260
10. A20 haploinsufficiency disturbs immune homeostasis and drives the transformation of lymphocytes with permissive antigen receptors.  |  Schultheiß, C., et al. 2024. Sci Adv. 10: eadl3975. PMID: 39167656

The preferred A20/TNFAIP3 antibody is A20/TNFAIP3 Antibody (A-12): sc-166692.