A1Up Antibody (A333): sc-136145
- A1Up Antibody (A333) is a mouse monoclonal IgG1 κ A1Up antibody, cited in 10 publications, provided at 200 µg/ml
- raised against a recombinant protein corresponding to the UBA domain of A1Up (UBA domain) of human origin
- A1Up Antibody (A333) is recommended for detection of A1Up (UBA domain) of mouse, rat and human origin by WB, IP and IF
- Anti-A1Up Antibody (A333) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for A1Up Antibody (A333) for WB applications. These reagents are now offered in bundles with A1Up Antibody (A333) (see ordering information below).
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A1Up Antibody (A333) is a mouse monoclonal IgG1 kappa light chain antibody that detects A1Up protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). A1Up (A333) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. A1Up, also known as Ataxin-1 ubiquitin-like-interacting protein or UBQLN4, is a 601 amino acid protein that plays a crucial role in cellular processes by localizing to both cytoplasm and nucleus, where A1Up associates with endoplasmic reticulum (ER). This localization enables A1Up to interact with proteins destined for ER, facilitating proper folding and processing. A1Up shows high expression in kidney, pancreas, heart, brain, and skeletal muscle, with lower levels in liver, lung, and placenta. A1Up functions as a homodimer that binds to signal sequences on proteins targeted to ER. Additionally, A1Up links Ataxin-1 with ubiquitin/proteasome pathways, potentially aiding in formation of multimeric protein complexes within nucleus. A1Up contains one ubiquitin-like domain and one UBA domain, and undergoes phosphorylation in response to DNA damage, highlighting A1Up′s involvement in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
A1Up Antibody (A333) References:
- Identification of two paralogous regions mapping to the short and long arms of human chromosome 2 comprising LIS1 pseudogenes. | Fogli, A., et al. 1999. Cytogenet Cell Genet. 86: 225-32. PMID: 10575211
- Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like nuclear protein. | Davidson, JD., et al. 2000. Hum Mol Genet. 9: 2305-12. PMID: 11001934
- Molecular cloning of a novel ubiquitin-like protein, UBIN, that binds to ER targeting signal sequences. | Matsuda, M., et al. 2001. Biochem Biophys Res Commun. 280: 535-40. PMID: 11162551
- The effects of the polyglutamine repeat protein ataxin-1 on the UbL-UBA protein A1Up. | Riley, BE., et al. 2004. J Biol Chem. 279: 42290-301. PMID: 15280365
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
- A novel connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of connexin43. | Li, X., et al. 2008. J Biol Chem. 283: 5748-59. PMID: 18079109
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A1Up Antibody (A333) | sc-136145 | 200 µg/ml | $316.00 | |||
A1Up Antibody (A333): m-IgG Fc BP-HRP Bundle | sc-528801 | 200 µg Ab; 10 µg BP | $354.00 | |||
A1Up Antibody (A333): m-IgGκ BP-HRP Bundle | sc-521332 | 200 µg Ab, 40 µg BP | $354.00 | |||
A1Up Antibody (A333): m-IgG1 BP-HRP Bundle | sc-543138 | 200 µg Ab; 20 µg BP | $354.00 | |||
A1Up Antibody (A333) AC | sc-136145 AC | 500 µg/ml, 25% agarose | $416.00 | |||
A1Up Antibody (A333) HRP | sc-136145 HRP | 200 µg/ml | $316.00 | |||
A1Up Antibody (A333) FITC | sc-136145 FITC | 200 µg/ml | $330.00 | |||
A1Up Antibody (A333) PE | sc-136145 PE | 200 µg/ml | $343.00 | |||
A1Up Antibody (A333) Alexa Fluor® 488 | sc-136145 AF488 | 200 µg/ml | $357.00 | |||
A1Up Antibody (A333) Alexa Fluor® 546 | sc-136145 AF546 | 200 µg/ml | $357.00 | |||
A1Up Antibody (A333) Alexa Fluor® 594 | sc-136145 AF594 | 200 µg/ml | $357.00 | |||
A1Up Antibody (A333) Alexa Fluor® 647 | sc-136145 AF647 | 200 µg/ml | $357.00 | |||
A1Up Antibody (A333) Alexa Fluor® 680 | sc-136145 AF680 | 200 µg/ml | $357.00 | |||
A1Up Antibody (A333) Alexa Fluor® 790 | sc-136145 AF790 | 200 µg/ml | $357.00 |