A-Raf Antibody (A-5): sc-166771

A-Raf Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects A-Raf protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-A-Raf antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. A-Raf, a member of the Raf gene family of serine/threonine protein kinases, plays a crucial role in cell signaling pathways that regulate cell growth and differentiation. A-Raf (A-5) antibody enables research into cancer biology, where aberrant activation of A-Raf can lead to uncontrolled cell proliferation and tumorigenesis. A-Raf is predominantly expressed in urogenital tissues and kidney, with lower expression levels in brain, highlighting potential roles in organ-specific signaling pathways. A-Raf kinase activity maintains tight regulation, and interaction with other signaling molecules, such as ERK/MAP kinases, underscores significance in mediating cellular responses to growth factors and stress signals. A-Raf (A-5) monoclonal antibody supports research into signal transduction mechanisms and A-Raf implications in various physiological and pathological processes.

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A-Raf Antibody (A-5) References:

1. A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes.  |  Provot, S., et al. 2008. Mol Cell Biol. 28: 344-57. PMID: 17967876
2. Interleukin 2 induces tyrosine phosphorylation and activation of p72-74 Raf-1 kinase in a T-cell line.  |  Turner, B., et al. 1991. Proc Natl Acad Sci U S A. 88: 1227-31. PMID: 1996324
3. Protein kinase cascades in meiotic and mitotic cell cycle control.  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
4. A Raf-induced allosteric transition of KSR stimulates phosphorylation of MEK.  |  Brennan, DF., et al. 2011. Nature. 472: 366-9. PMID: 21441910
5. Mutational activation of c-raf-1 and definition of the minimal transforming sequence.  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
6. Requirement of B-Raf, C-Raf, and A-Raf for the growth and survival of mouse embryonic stem cells.  |  Guo, W., et al. 2013. Exp Cell Res. 319: 2801-11. PMID: 24051329
7. Expression of the c-raf protooncogene in human hematopoietic cells and cell lines.  |  Sariban, E., et al. 1987. Blood. 69: 1437-40. PMID: 2436688
8. Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
9. Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
10. Allosteric and ATP-Competitive MEK-Inhibition in a Novel Spitzoid Melanoma Model with a RAF- and Phosphorylation-Independent Mutation.  |  Hegedüs, L., et al. 2021. Cancers (Basel). 13: PMID: 33669371
11. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
12. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus.  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-A-Raf Antibody (A-5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-A-Raf Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

A-Raf Antibody (A-5) Specifications

Alternate Product Name: ARAF

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 141-210

Epitope Target: A-Raf

Epitope Species: human

Product Reactivity/Detection: A-Raf

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for A-Raf Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin