60 kDa Ro/SSA Antibody (AA-3): sc-100844

60 kDa Ro/SSA Antibody (AA-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects 60 kDa Ro/SSA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-60 kDa Ro/SSA antibody (AA-3) is available as a non-conjugated format, making it versatile for various research applications. 60 kDa Ro/SSA protein, a ribonucleoprotein, plays a crucial role in immune response and is associated with several autoimmune conditions, including primary Sjögren syndrome and systemic lupus erythematosus. 60 kDa Ro/SSA (AA-3) antibody recognizes autoantibodies present in a substantial percentage of patients with these diseases, serving as a biomarker for diagnosis and monitoring. 60 kDa Ro/SSA protein is located primarily in the nucleus, involved in RNA processing and stability, highlighting importance in maintaining cellular function and integrity. Ro/SSA particle comprises a single immunoreactive protein noncovalently bound to one of four small RNA molecules, and this interaction is essential for RNA metabolism. Genes encoding larger and smaller Ro/SSA proteins are mapped to human chromosomes 11p15.5 and 1q31, respectively, indicating genetic significance in autoimmune disorders. Presence of antibodies against Ro/SSA is critical in autoimmune disease pathogenesis, making 60 kDa Ro/SSA (AA-3) monoclonal antibody invaluable for studying these conditions.

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60 kDa Ro/SSA Antibody (AA-3) References:

1. Routine immunofluorescence detection of Ro/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro/SS-A.  |  Pollock, W. and Toh, BH. 1999. J Clin Pathol. 52: 684-7. PMID: 10655991
2. Autoantibody to the leucine zipper region of 52 kDa Ro/SSA binds native 60 kDa Ro/SSA: identification of a tertiary epitope with components from 60 kDa Ro/SSA and 52 kDa Ro/SSA.  |  Kurien, BT., et al. 2001. Scand J Immunol. 53: 268-76. PMID: 11251884
3. 52-kDa Ro/SSA epitopes preferentially recognized by antibodies from mothers of children with neonatal lupus and congenital heart block.  |  Fritsch, C., et al. 2006. Arthritis Res Ther. 8: R4. PMID: 16356190
4. Differential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjögren's syndrome.  |  Barcellos, KS., et al. 2007. J Rheumatol. 34: 1283-92. PMID: 17552056
5. Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control.  |  Hogg, JR. and Collins, K. 2007. Genes Dev. 21: 3067-72. PMID: 18056422
6. B cell apotopes of the 60-kDa Ro/SSA and La/SSB autoantigens.  |  Reed, JH., et al. 2008. J Autoimmun. 31: 263-7. PMID: 18511235
7. Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes.  |  Itoh, K., et al. 1991. J Clin Invest. 87: 177-86. PMID: 1985094
8. Genomic structure and amino acid sequence domains of the human La autoantigen.  |  Chambers, JC., et al. 1988. J Biol Chem. 263: 18043-51. PMID: 3192525
9. Autoimmune sera react with multiple epitopes on recombinant 52 and 60 kDa Ro(SSA) proteins.  |  McCauliffe, DP., et al. 1994. J Rheumatol. 21: 1073-80. PMID: 7523671
10. Human 60-kDa SS-A/Ro ribonucleoprotein autoantigen gene (SSA2) localized to 1q31 by fluorescence in situ hybridization.  |  Chan, EK., et al. 1994. Genomics. 23: 298-300. PMID: 7829096
11. The mapping of the human 52-kD Ro/SSA autoantigen gene to human chromosome 11, and its polymorphisms.  |  Frank, MB., et al. 1993. Am J Hum Genet. 52: 183-91. PMID: 8094596
12. Anti-Ro/SSA associated recurrent annular erythema: autoimmune response to recombinant 60- and 52-kDa Ro/SSA proteins.  |  Miyagawa, S., et al. 1996. J Dermatol Sci. 12: 127-31. PMID: 8814544

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-60 kDa Ro/SSA Antibody (AA-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-60 kDa Ro/SSA Antibody (AA-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

60 kDa Ro/SSA Antibody (AA-3) Specifications

Alternate Product Name: 60 kDa Ro/SSA

Product Clone Name: AA-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-101

Epitope Target: 60 kDa Ro/SSA

Epitope Species: human

Product Reactivity/Detection: 60 kDa Ro/SSA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for 60 kDa Ro/SSA Antibody (AA-3): N/A