53BP2 Antibody (H-4): sc-398311
- 53BP2 Antibody (H-4) is a mouse monoclonal IgG2b κ 53BP2 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-19 at the N-terminus of 53BP2 of human origin
- 53BP2 Antibody (H-4) is recommended for detection of 53BP2 of human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-53BP2 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 53BP2 Antibody (H-4) for WB applications. These reagents are now offered in bundles with 53BP2 Antibody (H-4) (see ordering information below).
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53BP2 Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects 53BP2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-53BP2 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 53BP2 plays a crucial role in the cellular response to DNA damage, particularly in p53-mediated tumor suppression. 53BP2 interacts specifically with the central DNA-binding domain of wild-type p53, which is essential for function in regulating cell cycle and apoptosis. Notably, 53BP2 binding to p53 depends on p53 conformation, highlighting the importance of protein structure in mediating these critical interactions. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus may be found in the nucleus, suggesting a dual role in both cytoplasmic signaling and nuclear functions. 53BP2 has been shown to induce cell death by interacting with Bcl2 and NFkB p65, further emphasizing significance in the regulation of cell survival and apoptosis. 53BP2 monoclonal antibody (H-4) serves as an invaluable tool for researchers studying the mechanisms of tumor suppression and cellular response to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
53BP2 Antibody (H-4) References:
- NF-kappaB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2. | Yang, JP., et al. 1999. Oncogene. 18: 5177-86. PMID: 10498867
- Aberrant overexpression of 53BP2 mRNA in lung cancer cell lines. | Mori, T., et al. 2000. FEBS Lett. 465: 124-8. PMID: 10631318
- Proapoptotic p53-interacting protein 53BP2 is induced by UV irradiation but suppressed by p53. | Lopez, CD., et al. 2000. Mol Cell Biol. 20: 8018-25. PMID: 11027272
- Hepatitis C virus core protein interacts with p53-binding protein, 53BP2/Bbp/ASPP2, and inhibits p53-mediated apoptosis. | Cao, Y., et al. 2004. Biochem Biophys Res Commun. 315: 788-95. PMID: 14985081
- 53BP2 induces apoptosis through the mitochondrial death pathway. | Kobayashi, S., et al. 2005. Genes Cells. 10: 253-60. PMID: 15743414
- TP53BP2 locus is associated with gastric cancer susceptibility. | Ju, H., et al. 2005. Int J Cancer. 117: 957-60. PMID: 15986435
- Effects of oncogenic mutations and DNA response elements on the binding of p53 to p53-binding protein 2 (53BP2). | Tidow, H., et al. 2006. J Biol Chem. 281: 32526-33. PMID: 16887812
- miR-219a-5p Ameliorates Hepatic Ischemia/Reperfusion Injury via Impairing TP53BP2. | Xiao, Y., et al. 2019. Dig Dis Sci. 64: 2177-2186. PMID: 30796685
- TP53BP2 decreases cell proliferation and induces autophagy in neuroblastoma cell lines. | Pang, Y., et al. 2019. Oncol Lett. 17: 4976-4984. PMID: 31186708
- Distinct residues of human p53 implicated in binding to DNA, simian virus 40 large T antigen, 53BP1, and 53BP2. | Thukral, SK., et al. 1994. Mol Cell Biol. 14: 8315-21. PMID: 7969167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
53BP2 Antibody (H-4) | sc-398311 | 200 µg/ml | $322.00 | |||
53BP2 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-530753 | 200 µg Ab; 10 µg BP | $361.00 | |||
53BP2 Antibody (H-4): m-IgGκ BP-HRP Bundle | sc-524288 | 200 µg Ab, 40 µg BP | $361.00 | |||
53BP2 Antibody (H-4): m-IgG2b BP-HRP Bundle | sc-550221 | 200 µg Ab; 10 µg BP | $361.00 | |||
53BP2 Antibody (H-4) AC | sc-398311 AC | 500 µg/ml, 25% agarose | $424.00 | |||
53BP2 Antibody (H-4) HRP | sc-398311 HRP | 200 µg/ml | $322.00 | |||
53BP2 Antibody (H-4) FITC | sc-398311 FITC | 200 µg/ml | $336.00 | |||
53BP2 Antibody (H-4) PE | sc-398311 PE | 200 µg/ml | $349.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 488 | sc-398311 AF488 | 200 µg/ml | $364.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 546 | sc-398311 AF546 | 200 µg/ml | $364.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 594 | sc-398311 AF594 | 200 µg/ml | $364.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 647 | sc-398311 AF647 | 200 µg/ml | $364.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 680 | sc-398311 AF680 | 200 µg/ml | $364.00 | |||
53BP2 Antibody (H-4) Alexa Fluor® 790 | sc-398311 AF790 | 200 µg/ml | $364.00 | |||
53BP2 (H-4) Neutralizing Peptide | sc-398311 P | 100 µg/0.5 ml | $69.00 |