53BP2 Antibody (DX54.10): sc-53861

53BP2 Antibody (DX54.10) is a mouse monoclonal IgG1 kappa light chain antibody that detects 53BP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-53BP2 antibody (DX54.10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 53BP2 protein plays a crucial role in cellular response to DNA damage, particularly in p53-mediated tumor suppression. 53BP2 interacts specifically with the central DNA-binding domain of wild-type p53, which is essential for function in regulating cell cycle and apoptosis. 53BP2 binds to the L2 loop of p53, a region often mutated in various cancers, highlighting importance in maintaining genomic stability. 53BP2 localization is also significant; while N-terminus is found in cytoplasm, C-terminus is likely localized in nucleus, suggesting a dual role in both cytoplasmic signaling and nuclear functions related to DNA repair and cell cycle regulation. 53BP2 has been shown to induce cell death by interacting with Bcl2 and NFkB p65, underscoring potential as a therapeutic target in cancer treatment.

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53BP2 Antibody (DX54.10) References:

1. NF-kappaB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2.  |  Yang, JP., et al. 1999. Oncogene. 18: 5177-86. PMID: 10498867
2. p53-interacting protein 53BP2 inhibits clonogenic survival and sensitizes cells to doxorubicin but not paclitaxel-induced apoptosis.  |  Ao, Y., et al. 2001. Oncogene. 20: 2720-5. PMID: 11420684
3. Expression of 53BP2 and ASPP2 proteins from TP53BP2 gene by alternative splicing.  |  Takahashi, N., et al. 2004. Biochem Biophys Res Commun. 315: 434-8. PMID: 14766226
4. miR-548d-3p/TP53BP2 axis regulates the proliferation and apoptosis of breast cancer cells.  |  Song, Q., et al. 2016. Cancer Med. 5: 315-24. PMID: 26663100
5. Sufentanil Protects the Liver from Ischemia/Reperfusion-Induced Inflammation and Apoptosis by Inhibiting ATF4-Induced TP53BP2 Expression.  |  Zhou, L., et al. 2021. Inflammation. 44: 1160-1174. PMID: 33751357
6. TP53BP2: Roles in suppressing tumorigenesis and therapeutic opportunities.  |  Huo, Y., et al. 2023. Genes Dis. 10: 1982-1993. PMID: 37492707
7. Higher TP53BP2 expression is associated with HBsAg loss in peginterferon-α-treated patients with chronic hepatitis B.  |  Guan, G., et al. 2024. J Hepatol. 80: 41-52. PMID: 37858684
8. Two cellular proteins that bind to wild-type but not mutant p53.  |  Iwabuchi, K., et al. 1994. Proc Natl Acad Sci U S A. 91: 6098-102. PMID: 8016121
9. The p53-binding protein 53BP2 also interacts with Bc12 and impedes cell cycle progression at G2/M.  |  Naumovski, L. and Cleary, ML. 1996. Mol Cell Biol. 16: 3884-92. PMID: 8668206
10. Structure of the p53 tumor suppressor bound to the ankyrin and SH3 domains of 53BP2.  |  Gorina, S. and Pavletich, NP. 1996. Science. 274: 1001-5. PMID: 8875926
11. p202, an interferon-inducible modulator of transcription, inhibits transcriptional activation by the p53 tumor suppressor protein, and a segment from the p53-binding protein 1 that binds to p202 overcomes this inhibition.  |  Datta, B., et al. 1996. J Biol Chem. 271: 27544-55. PMID: 8910340
12. Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.  |  Iwabuchi, K., et al. 1998. J Biol Chem. 273: 26061-8. PMID: 9748285

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-53BP2 Antibody (DX54.10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-53BP2 Antibody (DX54.10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

53BP2 Antibody (DX54.10) Specifications

Alternate Product Name: 53BP2

Product Clone Name: DX54.10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 691-1128

Epitope Target: 53BP2

Epitope Species: human

Product Reactivity/Detection: 53BP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for 53BP2 Antibody (DX54.10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred 53BP2 antibody is 53BP2 Antibody (H-4): sc-398311.