52 kDa Ro/SSA CRISPR Activation Plasmid (h): sc-400885-ACT. Western blot analysis of 52 kDa Ro/SSA expression in non-transfected control (A) and 52 kDa Ro/SSA CRISPR Activation Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with 52 kDa Ro/SSA (E-11): sc-48430. β-actin (C4): sc-47778 used as specificity and loading control.
52 kDa Ro/SSA CRISPR Plasmids (h)
- Target species: human; for corresponding mouse product, see 52 kDa Ro/SSA CRISPR Plasmids (m)
- CRISPR/Cas9 KO Plasmids consists of 52 kDa Ro/SSA-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using 52 kDa Ro/SSA Antibody (D-12): sc-25351
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
HDR Plasmid
Double Nickase Plasmids
CRISPR Activation Plasmids
Lentiviral Activation Particles
Recommended CRISPR and HDR Support Reagents
Recommended Control Products
SEE ALSO...
52 kDa Ro/SSA Antibodies for analysis of cellular responses to 52 kDa Ro/SSA CRISPR Products
Learn more about the CRISPR System
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details
Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 52 kDa Ro/SSA CRISPR/Cas9 Knockout (KO) Plasmid (h) consists of a pool of three plasmids each encoding the Cas9 nuclease and a 52 kDa Ro/SSA-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
- gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- For selection of cells containing a successful Cas9-induced DSB, co-transfection with 52 kDa Ro/SSA HDR Plasmid (h) (sc-400885-HDR) is recommended
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: 52 kDa Ro/SSA Antibody (D-12): sc-25351
See Protocol Online.
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Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 52 kDa Ro/SSA HDR Plasmid (h) is recommended for co-transfection with 52 kDa Ro/SSA CRISPR/Cas9 KO Plasmid (h)
- 52 kDa Ro/SSA HDR Plasmid (h) consists of a pool of 2-3 plasmids, each containing a homology-directed DNA repair (HDR) template corresponding to the cut sites generated by the 52 kDa Ro/SSA CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two 800 bp homology arms designed to specifically bind to the genomic DNA surrounding the corresponding Cas9-induced double strand DNA break site
- Each HDR Plasmid inserts a puromycin resistance gene to enable selection of stable knockout (KO) cells and an RFP (Red Fluorescent Protein) gene to visually verify transfection
- The puromycin resistance and RFP genes are flanked by two LoxP sites to allow for further processing by the Cre Vector sc-418923
See Protocol Online.
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Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 52 kDa Ro/SSA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- The paired gRNAs encoded by 52 kDa Ro/SSA Double Nickase Plasmid (h2) differ from the paired gRNAs in 52 kDa Ro/SSA Double Nickase Plasmid (h)
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: 52 kDa Ro/SSA Antibody (D-12): sc-25351
See Protocol Online.
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Description
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 52 kDa Ro/SSA CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- 52 kDa Ro/SSA CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: 52 kDa Ro/SSA Antibody (D-12): sc-25351
See Protocol Online.
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Description
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- 52 kDa Ro/SSA Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- 52 kDa Ro/SSA Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt. guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt. upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- The guide RNA in the 52 kDa Ro/SSA Lentiviral Activation Particles (h2) encodes a target-specific 20 nt guide RNA that differs from the guide RNA in 52 kDa Ro/SSA Lentiviral Activation Particles (h)
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: 52 kDa Ro/SSA Antibody (D-12): sc-25351
See Protocol Online.
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
52 kDa Ro/SSA CRISPR/Cas9 KO Plasmid (h) | sc-400885 | 20 µg | $397.00 | |||
52 kDa Ro/SSA HDR Plasmid (h) | sc-400885-HDR | 20 µg | $445.00 | |||
52 kDa Ro/SSA Double Nickase Plasmid (h) | sc-400885-NIC | 20 µg | $410.00 | |||
| Not Available | ||||||
52 kDa Ro/SSA Double Nickase Plasmid (h2) | sc-400885-NIC-2 | 20 µg | $410.00 | |||
| Not Available | ||||||
52 kDa Ro/SSA CRISPR Activation Plasmid (h) | sc-400885-ACT | 20 µg | $397.00 | |||
52 kDa Ro/SSA CRISPR Activation Plasmid (h2) | sc-400885-ACT-2 | 20 µg | $397.00 | |||
52 kDa Ro/SSA Lentiviral Activation Particles (h) | sc-400885-LAC | 200 µl | $455.00 | |||
| Not Available | ||||||
52 kDa Ro/SSA Lentiviral Activation Particles (h2) | sc-400885-LAC-2 | 200 µl | $455.00 | |||
| Not Available | ||||||