5-LO Antibody (A-9): sc-518175

5-LO Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects 5-lipoxygenase (5-LO) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). 5-LO plays a crucial role in the biosynthesis of leukotrienes, which are lipid mediators that significantly influence inflammatory responses, particularly in conditions such as asthma and allergic reactions. 5-LO is primarily expressed in polymorphonuclear leukocytes, macrophages, and mast cells, where 5-LO catalyzes the initial steps in leukotriene production. Notably, 5-LO cellular localization is dynamic and varies among different cell types; for instance, in activated blood polymorphonuclear leukocytes, 5-LO translocates from the cytosol to the nuclear envelope in a calcium-dependent manner. In alveolar macrophages, the majority of 5-LO is found in the nucleus, and upon activation, 5-LO binds to the nuclear membrane, a process that is influenced by various signaling molecules and post-translational modifications, including phosphorylation. This intracellular shuttling is essential for 5-LO function, as such movement allows for precise regulation of leukotriene synthesis in response to cellular signals, highlighting 5-LO importance in mediating inflammatory processes. Anti-5-LO antibody (A-9) serves as an invaluable tool for researchers studying the mechanisms of inflammation and the role of leukotrienes in various diseases.

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5-LO Antibody (A-9) References:

1. 5-lipoxygenase inhibitors and their anti-inflammatory activities.  |  Batt, DG. 1992. Prog Med Chem. 29: 1-63. PMID: 1475368
2. The 5-lipoxygenase pathway in arterial wall biology and atherosclerosis.  |  Lötzer, K., et al. 2005. Biochim Biophys Acta. 1736: 30-7. PMID: 16081317
3. Pharmacotherapy of diseases mediated by 5-lipoxygenase pathway eicosanoids.  |  Rubin, P. and Mollison, KW. 2007. Prostaglandins Other Lipid Mediat. 83: 188-97. PMID: 17481554
4. 5-Lipoxygenase-mediated endogenous DNA damage.  |  Jian, W., et al. 2009. J Biol Chem. 284: 16799-16807. PMID: 19390118
5. Exploring QSARs for 5-lipoxygenase (5-LO) inhibitory activity of 2-substituted 5-hydroxyindole-3-carboxylates by CoMFA and CoMSIA.  |  Zheng, J., et al. 2011. Chem Biol Drug Des. 78: 314-21. PMID: 21599857
6. 5-Lipoxygenase-activating protein rescues activity of 5-lipoxygenase mutations that delay nuclear membrane association and disrupt product formation.  |  Gerstmeier, J., et al. 2016. FASEB J. 30: 1892-900. PMID: 26842853
7. Inhibition of 5-Lipoxygenase in Hepatic Stellate Cells Alleviates Liver Fibrosis.  |  Pu, S., et al. 2021. Front Pharmacol. 12: 628583. PMID: 33679410
8. Blocking 5-LO pathway alleviates renal fibrosis by inhibiting the epithelial-mesenchymal transition.  |  Zhou, J., et al. 2021. Biomed Pharmacother. 138: 111470. PMID: 33721755
9. Helical remodeling augments 5-lipoxygenase activity in the synthesis of proinflammatory mediators.  |  Gallegos, EM., et al. 2022. J Biol Chem. 298: 102282. PMID: 35863431
10. Pharmacogenetics of the 5-lipoxygenase pathway in asthma.  |  Silverman, E., et al. 1998. Clin Exp Allergy. 28 Suppl 5: 164-70; discussion 171-3. PMID: 9988463

The preferred 5-LO antibody is 5-LO Antibody (B-3): sc-518174.