20S Proteasome β5 Antibody (A-10): sc-393931

20S Proteasome β5 Antibody (A-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects 20S Proteasome β5 (PSMB5) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PSMB5 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSMB5 plays a crucial role in cellular protein homeostasis by degrading unnecessary or damaged proteins through proteolysis. PSMB5 functions within a hollow structure that allows substrate protein entry, which are then processed by catalytic β subunits. Proper functioning of PSMB5 is vital for maintaining cellular health, as accumulation of misfolded or damaged proteins can lead to cellular dysfunction and disease. Anti-PSMB5 antibody (A-10) enables researchers to gain valuable insights into proteolytic pathways fundamental to cellular regulation and disease mechanisms.

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20S Proteasome β5 Antibody (A-10) References:

1. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
2. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
3. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
4. Computational modeling of the potential interactions of the proteasome beta5 subunit and catechol-O-methyltransferase-resistant EGCG analogs.  |  Kanwar, J., et al. 2010. Int J Mol Med. 26: 209-15. PMID: 20596600
5. In silico identification of 20S proteasome-β5 subunit inhibitors using structure-based virtual screening.  |  El Yaagoubi, OM., et al. 2024. J Biomol Struct Dyn. 42: 6165-6173. PMID: 37403265
6. Structural comparison of human and Plasmodium proteasome β5 subunits: informing selective inhibitor design for anti-malaria agents.  |  Yasir, M., et al. 2025. Malar J. 24: 21. PMID: 39838389
7. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
8. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
9. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
10. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
11. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
12. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome β5 Antibody (A-10) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome β5 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome β5 Antibody (A-10) Specifications

Alternate Product Name: 20S Proteasome β5

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 217-263

Epitope Target: 20S Proteasome β5

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome β5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome β5 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin