20S Proteasome α3 Antibody (A-9): sc-166205

20S Proteasome α3 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects 20S Proteasome α3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome α3 antibody (A-9) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. 20S Proteasome α3 plays a crucial role in cellular protein homeostasis by degrading misfolded, damaged, or unneeded proteins, thus preventing the accumulation of potentially toxic protein aggregates. This process is vital for maintaining cellular health and regulating various biological processes, including the cell cycle, apoptosis, and responses to oxidative stress. The 20S proteasome core, composed of multiple subunits, forms a cylindrical structure that houses the catalytic sites for protein degradation, while regulatory particles control substrate entry, ensuring that only appropriate proteins are processed. Understanding 20S Proteasome α3 function is essential for elucidating its role in various diseases, including cancer and neurodegenerative disorders, where proteasome activity may be disrupted.

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20S Proteasome α3 Antibody (A-9) References:

1. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
2. Diagnostic importance of anti-proteasome antibodies.  |  Feist, E., et al. 2000. Int Arch Allergy Immunol. 123: 92-7. PMID: 11014975
3. Phosphorylation of 20S proteasome alpha subunit C8 (alpha7) stabilizes the 26S proteasome and plays a role in the regulation of proteasome complexes by gamma-interferon.  |  Bose, S., et al. 2004. Biochem J. 378: 177-84. PMID: 14583091
4. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
5. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
6. Gel-based chemical cross-linking analysis of 20S proteasome subunit-subunit interactions in breast cancer.  |  Song, H., et al. 2016. J Huazhong Univ Sci Technolog Med Sci. 36: 564-570. PMID: 27465334
7. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
8. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
9. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
10. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
11. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
12. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome α3 Antibody (A-9) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome α3 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome α3 Antibody (A-9) Specifications

Alternate Product Name: 20S Proteasome α3

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 131-255

Epitope Target: 20S Proteasome α3

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome α3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome α3 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin