20S Proteasome α2 Antibody (B-4): sc-377148

20S Proteasome α2 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects 20S Proteasome α2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome α2 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 20S Proteasome α2 (B-4) monoclonal antibody helps study the 20S proteasome, which plays a crucial role in cellular protein homeostasis by degrading misfolded, damaged, or unneeded proteins, thus preventing the accumulation of potentially toxic protein aggregates. This process is vital for maintaining cellular health and regulating various cellular processes, including the cell cycle, apoptosis, and responses to oxidative stress. The 20S proteasome is composed of a core particle that forms a cylindrical structure, allowing for the selective entry of substrates through its openings, where the catalytic β subunits facilitate the proteolytic degradation of proteins. Understanding the function of the 20S proteasome and its subunits is essential for elucidating its role in various diseases, including cancer and neurodegenerative disorders, where proteasome activity may be altered.

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20S Proteasome α2 Antibody (B-4) References:

1. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
2. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
3. Mechanism of direct degradation of IkappaBalpha by 20S proteasome.  |  Alvarez-Castelao, B. and Castaño, JG. 2005. FEBS Lett. 579: 4797-802. PMID: 16098527
4. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
5. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
6. Gel-based chemical cross-linking analysis of 20S proteasome subunit-subunit interactions in breast cancer.  |  Song, H., et al. 2016. J Huazhong Univ Sci Technolog Med Sci. 36: 564-570. PMID: 27465334
7. Hsp70 and Hsp110 Chaperones Promote Early Steps of Proteasome Assembly.  |  Matias, AC., et al. 2022. Biomolecules. 13: PMID: 36671396
8. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
9. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
10. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
11. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
12. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome α2 Antibody (B-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome α2 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome α2 Antibody (B-4) Specifications

Alternate Product Name: 20S Proteasome α2

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 45-79

Epitope Target: 20S Proteasome α2

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome α2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome α2 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin