Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20S Proteasome α8 CRISPR/Cas9 KO Plasmid (h): sc-414520
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 20S Proteasome α8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the 20S Proteasome α8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: 20S Proteasome α8 Antibody (N-96): sc-130488
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
20S Proteasome α8 CRISPR/Cas9 KO Plasmid (h) | sc-414520 | 20 µg | $397.00 |
Overview
PSMA8 encodes the 20S proteasome alpha 8 subunit, a component of the proteasome core that contributes to regulated protein turnover and peptide generation through the ubiquitin–proteasome system. By supporting proteasome assembly and proteolytic capacity, PSMA8 influences cellular proteostasis, cell-cycle progression, and stress-adaptive responses linked to DNA damage signaling and antigen processing. Proteasome pathway perturbations can reshape signaling networks such as NF-κB and apoptosis cascades by altering the stability of key regulatory proteins. Dysregulated proteasome function is broadly relevant to disease biology, including cancer-associated proteostasis dependencies and immune-related phenotypes driven by altered protein degradation and peptide presentation.
20S Proteasome α8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PSMA8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PSMA8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PSMA8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish 20S Proteasome α8 protein expression.
This CRISPR knockout system enables efficient generation of PSMA8-deficient cell models for investigation of 20S Proteasome α8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PSMA8 exon(s) critical for 20S Proteasome α8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PSMA8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by 20S Proteasome α8 CRISPR/Cas9 KO Plasmid (h) and 20S Proteasome α8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PSMA8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by 20S Proteasome α8 HDR Plasmid (h) and 20S Proteasome α8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PSMA8 homology arms to support homology-directed repair at defined PSMA8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.