20S Proteasome alpha 8 Antibody (N-96) recommended for detection of 20S Proteasome α8 of human origin by WB and IP

20S Proteasome α8 Antibody (N-96): sc-130488

20S Proteasome α8 Antibody (N-96) is rated 4.0 out of 5 by 1.
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Datasheets

    • 20S Proteasome α8 Antibody (N-96) is a mouse monoclonal IgG1 (kappa light chain) provided at 50 µg/0.5 ml
    • raised against recombinant 20S Proteasome α8 of human origin
    • recommended for detection of 20S Proteasome α8 of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using 20S Proteasome α8 (N-96): sc-130488 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust band seen in WB using K Robust band seen in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2013-01-11
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