20S Proteasome α7/α8 Antibody (B-4): sc-166761

20S Proteasome α7/α8 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects 20S Proteasome α7 in mouse and rat samples, as well as 20S Proteasome α8 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome α7/α8 antibody (B-4) is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The proteasome maintains cellular homeostasis by degrading unnecessary or damaged proteins, a process essential for regulating cellular functions, including cell cycle, signaling pathways, and responses to oxidative stress. The 20S Proteasome core comprises multiple subunits forming a cylindrical structure, enabling selective substrate entry into its catalytic chamber. This arrangement ensures efficient protein breakdown while maintaining tight regulation to prevent harmful protein accumulation within cells. Understanding proteasome dynamics and subunit functions helps reveal their role in health and disease, making 20S Proteasome α7/α8 (B-4) monoclonal antibody valuable for studying proteolytic pathways and their biological implications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

20S Proteasome α7/α8 Antibody (B-4) References:

1. Inhibition of protein synthesis by the nonstructural proteins NS4A and NS4B of hepatitis C virus.  |  Florese, RH., et al. 2002. Virus Res. 90: 119-31. PMID: 12457968
2. Effects of the isoform-specific characteristics of ATF6 alpha and ATF6 beta on endoplasmic reticulum stress response gene expression and cell viability.  |  Thuerauf, DJ., et al. 2007. J Biol Chem. 282: 22865-78. PMID: 17522056
3. Polymorphisms of ATF6B Are Potentially Associated With FEV1 Decline by Aspirin Provocation in Asthmatics.  |  Park, TJ., et al. 2014. Allergy Asthma Immunol Res. 6: 142-8. PMID: 24587951
4. The unfolded protein response genes in human osteoarthritic chondrocytes: PERK emerges as a potential therapeutic target.  |  Li, YH., et al. 2016. Arthritis Res Ther. 18: 172. PMID: 27435272
5. Overlapping and differential functions of ATF6α versus ATF6β in the mouse heart.  |  Correll, RN., et al. 2019. Sci Rep. 9: 2059. PMID: 30765833
6. The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity.  |  Nguyen, DT., et al. 2021. Sci Rep. 11: 13086. PMID: 34158584
7. Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells.  |  Saghaleyni, R., et al. 2022. Cell Rep. 39: 110936. PMID: 35705050
8. Salivary proteins offer insights into keratinocyte death during aphthous stomatitis. A case-crossover study.  |  Cofré-Leiva, C., et al. 2023. BMC Oral Health. 23: 279. PMID: 37170213
9. Genetic associations in ankylosing spondylitis: circulating proteins as drug targets and biomarkers.  |  Zhang, Y., et al. 2024. Front Immunol. 15: 1394438. PMID: 38835753
10. Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors.  |  Yoshida, H., et al. 1998. J Biol Chem. 273: 33741-9. PMID: 9837962

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome α7/α8 Antibody (B-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome α7/α8 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome α7/α8 Antibody (B-4) Specifications

Alternate Product Name: 20S Proteasome α7/α8

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-248

Epitope Target: 20S Proteasome α7

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome α7 of mouse, rat and human origin, and 20S Proteasome α8 of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome α7/α8 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin