Date published: 2025-12-14
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15-LO Antibody (B-7) - Western Blotting - Image 382215
15-LO Antibody (B-7): sc-133085. Western blot analysis of 15-LO expression in NIH/3T3 (A), c4 (B), AMJ2-C8 (C), EOC 20 (D), MCF7 (E) and PC-12 (F) whole cell lysates.
15-LO Antibody (B-7): sc-133085. Western blot analysis of 15-LO expression in NIH/3T3 (A), c4 (B), AMJ2-C8 (C), EOC 20 (D), MCF7 (E) and PC-12 (F) whole cell lysates.

15-LO Antibody (B-7): sc-133085

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Datasheets
  • 15-LO Antibody (B-7) is a mouse monoclonal IgG1 κ 15-LO antibody, cited in 23 publications, provided at 200 µg/ml
  • raised against acids 428-662 mapping at the C-terminus of 15-LO of human origin
  • 15-LO Antibody (B-7) is recommended for detection of 15-LO and leukocyte-, platelet- and epidermal-type 12-LO of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-15-LO Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 15-LO Antibody (B-7) for WB applications. These reagents are now offered in bundles with 15-LO Antibody (B-7) (see ordering information below).

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Research Information

SEE ALSO...

Additional LO Antibodies including 12-LO, 15-LO, 15-LO2 and 5-LO

15-LO Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects 15-LO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-15-LO antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 15-LO protein, also known as Arachidonate 15-Lipoxygenase, plays a crucial role in arachidonic acid metabolism, leading to bioactive lipid mediator production involved in various physiological processes, including inflammation, cell growth, and apoptosis. 15-LO enzyme is particularly important in cardiovascular health, as 15-LO is implicated in atherosclerosis pathogenesis and other inflammatory diseases. 15-LO expression regulation is tightly controlled and varies across different tissues and cell types, with cytokines such as interleukin-4 and interleukin-13 being key players in transcriptional activation. 15-LO type 1 overexpression has been linked to prostate cancer progression, where 15-LO modulates insulin-like growth factor 1 receptor signaling, highlighting potential as a therapeutic target. 15-LO influence on both normal physiological functions and pathological conditions underscores significance in biomedical research and therapeutic development.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

15-LO Antibody (B-7) References:

  1. Overexpression of platelet-type 12-lipoxygenase promotes tumor cell survival by enhancing alpha(v)beta(3) and alpha(v)beta(5) integrin expression.  |  Pidgeon, GP., et al. 2003. Cancer Res. 63: 4258-67. PMID: 12874035
  2. 15-lipoxygenase-1 expression upregulates and activates insulin-like growth factor-1 receptor in prostate cancer cells.  |  Kelavkar, UP. and Cohen, C. 2004. Neoplasia. 6: 41-52. PMID: 15068670
  3. Transcriptional regulation of 15-lipoxygenase expression by promoter methylation.  |  Liu, C., et al. 2004. Exp Cell Res. 297: 61-7. PMID: 15194425
  4. Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions.  |  Rásó, E., et al. 2004. Melanoma Res. 14: 245-50. PMID: 15305153
  5. Aspirin enhances the sensitivity of human platelet 12-lipoxygenase to inhibition by 15-HETE, an endogenous regulator.  |  Fletcher-Cieutat, M., et al. 1985. Prostaglandins Leukot Med. 18: 255-9. PMID: 3925465

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

15-LO Antibody (B-7)

sc-133085
200 µg/ml
$316.00

15-LO Antibody (B-7): m-IgG Fc BP-HRP Bundle

sc-528744
200 µg Ab; 10 µg BP
$354.00

15-LO Antibody (B-7): m-IgGκ BP-HRP Bundle

sc-521227
200 µg Ab, 40 µg BP
$354.00

15-LO Antibody (B-7): m-IgG1 BP-HRP Bundle

sc-543108
200 µg Ab; 20 µg BP
$354.00

15-LO Antibody (B-7) AC

sc-133085 AC
500 µg/ml, 25% agarose
$416.00

15-LO Antibody (B-7) HRP

sc-133085 HRP
200 µg/ml
$316.00

15-LO Antibody (B-7) FITC

sc-133085 FITC
200 µg/ml
$330.00

15-LO Antibody (B-7) PE

sc-133085 PE
200 µg/ml
$343.00

15-LO Antibody (B-7) Alexa Fluor® 488

sc-133085 AF488
200 µg/ml
$357.00

15-LO Antibody (B-7) Alexa Fluor® 546

sc-133085 AF546
200 µg/ml
$357.00

15-LO Antibody (B-7) Alexa Fluor® 594

sc-133085 AF594
200 µg/ml
$357.00

15-LO Antibody (B-7) Alexa Fluor® 647

sc-133085 AF647
200 µg/ml
$357.00

15-LO Antibody (B-7) Alexa Fluor® 680

sc-133085 AF680
200 µg/ml
$357.00

15-LO Antibody (B-7) Alexa Fluor® 790

sc-133085 AF790
200 µg/ml
$357.00

稀释比例是多少

Asked by: MILK
Thank you for your question. Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Answered by: Technical Service SH
Date published: 2025-08-07

I would like to use this antibody to perform flow cytometry on both mouse and human samples. I can't find any references showing it's use in flow cytometry, does it work for this application?

Asked by: Snoble
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2022-08-08

I have a question. Does this antibody react to both Alox12 (platelet type 12-LO) and Alox15 (white blood cell type 12-LO (12/15-LO)) in mice? What is the recommended dilution ratio when staining with frozen sections?

Asked by: Tachi
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hi, It says that this antibody detect platelet and leukocyte 12 lo. Does that mean that it detect by IF ALOX 15 and ALOX12

Asked by: MiJi
Thank you for your question. Yes, this antibody can detect 15-LO and leukocyte-, platelet- and epidermal-type 12-LO by immunofluorescence.
Answered by: Tech Service
Date published: 2019-12-04

I would like to use this antibody to perform immunoprecipitation, but couldn't find any references for that. Does it really work? Thanks!

Asked by: Alexa
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-02-10

I'd like to use this antibody to detect the protein 15-LO, in other words, 15-LOX-1. So I wonder if this antibody can also be able to detect 15-LO2 which would be bad for our specific research. Thanks!

Asked by: victor0gyz
Thank you for your question. This mouse monoclonal antibody is specific for detection of 15-LO and is not expected to cross-react with 15-LO2. We have not experimentally validated this, but based on the epitope the antibody is specific for 15-LO.
Answered by: Technical Support
Date published: 2018-11-22

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-133085: 15-LO (B-7) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from WB doesn't work for human or mouse samplesTried this antibody for both mouse and human epithelial cell lysates and had no bands. Another 15-LO from another company, I got beautiful bands. I'll have to go back to the previous company
Date published: 2018-10-11
Rated 4 out of 5 by from Works as advertisedI used a free sample aliquot and it works as expected.
Date published: 2018-01-02
Rated 2 out of 5 by from A signal of a doublet?I got this antibody as a free sample to detect endogenous 15-LO in human placenta lysates. I see a doublet above the predicted 75kDa weight and some other bands above 100kDa and above 150kDa. Not sure if the doublet bands above 75kda are the right ones.
Date published: 2017-10-20
Rated 5 out of 5 by from western blotsWe're very happy with the antibodies. Our regular application is in western blots with mouse kidney extract, We use it in dilution 1:800.
Date published: 2017-07-22
Rated 5 out of 5 by from 15-LO (B-7): sc-133085I tried this antibody one month ago.It was clear and nice in western blot.
Date published: 2017-02-08
Rated 5 out of 5 by from Works as expected in WBThis 15-LO monoclonal antibody gives satisfactory bands for WB on NIH/3T3 and KNRK whole cell lysates at a 1:200 dilutions
Date published: 2017-01-27
Rated 5 out of 5 by from Satisfactory Western Blot data of 15Satisfactory Western Blot data of 15-LO expression in HEK293 cells transfected with human 15-LO expression vector and HEK923 cells . -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Produced positive Western Blot data of 15Produced positive Western Blot data of 15-LO expression in human PBL, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2013-04-10
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15-LO Antibody (B-7) is rated 4.0 out of 5 by 8.
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