14-3-3 ε Antibody (F-3): sc-393177

14-3-3 ε Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects 14-3-3 ε in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 ε antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 ε plays a crucial role in cellular signaling by regulating various processes, including apoptosis, mitogenic signaling, and cell-cycle checkpoints, which are particularly relevant in cancer biology. 14-3-3 ε belongs to a family of seven isoforms, including 14-3-3 β, γ, ε, ζ, η, θ, and Σ, that function as dimeric signaling intermediates. Each dimer provides two binding sites for ligand proteins, facilitating interactions that may not occur otherwise. The ligands typically share a consensus binding motif and are often phosphorylated on serine or threonine residues, which is essential for their interaction with 14-3-3 proteins. Through regulating these interactions, 14-3-3 ε can influence a wide range of cellular activities, including protein sequestration in the cytoplasm, modulation of enzymatic activity, and prevention of protein degradation. Alterations in 14-3-3 ε expression or function can lead to significant pathogenic consequences, underscoring its importance in maintaining cellular homeostasis and potential as a therapeutic target in cancer and other diseases.

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14-3-3 ε Antibody (F-3) References:

1. 14-3-3 epsilon modulates the stimulated secretion of endopeptidase 24.15.  |  Carreño, FR., et al. 2005. J Neurochem. 93: 10-25. PMID: 15773901
2. Inhibitory interaction of the 14-3-3{epsilon} protein with isoform 4 of the plasma membrane Ca(2+)-ATPase pump.  |  Rimessi, A., et al. 2005. J Biol Chem. 280: 37195-203. PMID: 16126729
3. 14-3-3 epsilon dynamically interacts with key components of mitogen-activated protein kinase signal module for selective modulation of the TNF-alpha-induced time course-dependent NF-kappaB activity.  |  Zuo, S., et al. 2010. J Proteome Res. 9: 3465-78. PMID: 20462248
4. Clinical implication of 14-3-3 epsilon expression in gastric cancer.  |  Leal, MF., et al. 2012. World J Gastroenterol. 18: 1531-7. PMID: 22509086
5. 14-3-3 epsilon prevents G2/M transition of fertilized mouse eggs by binding with CDC25B.  |  Cui, C., et al. 2014. BMC Dev Biol. 14: 33. PMID: 25059436
6. Pretreatment 14-3-3 epsilon level is predictive for advanced extranodal NK/T cell lymphoma therapeutic response to asparaginase-based chemotherapy.  |  Qiu, Y., et al. 2017. Proteomics Clin Appl. 11: PMID: 27774748
7. Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development.  |  Keicher, J., et al. 2017. Elife. 6: PMID: 28422008
8. 14-3-3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele.  |  He, QQ., et al. 2019. Andrologia. 51: e13275. PMID: 30950109
9. Activation of innate immunity by 14-3-3 ε, a new potential alarmin in osteoarthritis.  |  Millerand, M., et al. 2020. Osteoarthritis Cartilage. 28: 646-657. PMID: 32173627
10. Identification of 14-3-3 epsilon as a regulator of the neural apoptotic pathway for chronic-stress-induced depression.  |  Zhao, Y., et al. 2021. iScience. 24: 102043. PMID: 33537655

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-14-3-3 ε Antibody (F-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-14-3-3 ε Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

14-3-3 ε Antibody (F-3) Specifications

Alternate Product Name: 14-3-3 epsilon

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 136-175

Epitope Target: 14-3-3 ε

Epitope Species: human

Product Reactivity/Detection: 14-3-3 ε

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for 14-3-3 ε Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred 14-3-3 ε antibody is 14-3-3 ε Antibody (8C3): sc-23957.