11β-HSD1 Antibody (D-5): sc-518168
- 11β-HSD1 Antibody (D-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 90-112 of 11β-HSD1 of mouse origin
- recommended for detection of 11β-HSD1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-11 beta-HSD1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 11β-HSD1 Antibody (D-5) for WB applications. These reagents are now offered in bundles with 11β-HSD1 Antibody (D-5) (see ordering information below).
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SEE ALSO...
11β-HSD1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects 11β-HSD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-11β-HSD1 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) plays a critical role in glucocorticoid hormone action by catalyzing the conversion of inactive cortisone to active cortisol, which is essential for various physiological processes, including metabolism and immune response. 11β-HSD1 is predominantly expressed in the liver, where 11β-HSD1 influences glucose homeostasis and may contribute to the pathogenesis of metabolic disorders such as insulin resistance and obesity. Additionally, glycosylation of 11β-HSD1 can affect enzymatic activity and stability, thereby impacting regulatory functions in target tissues. Understanding 11β-HSD1 dynamics and interactions with other proteins is crucial for elucidating roles in health and disease, making 11β-HSD1 (D-5) monoclonal antibody an invaluable research tool for studying these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
11β-HSD1 Antibody (D-5) References:
- 11 beta-Hydroxysteroid dehydrogenase. | Stewart, PM. and Krozowski, ZS. 1999. Vitam Horm. 57: 249-324. PMID: 10232052
- Expression of 11 beta-hydroxysteroid dehydrogenase in early pregnancy: implications in human trophoblast-endometrial interactions. | Arcuri, F., et al. 1999. Semin Reprod Endocrinol. 17: 53-61. PMID: 10406076
- Functional expression, characterization, and purification of the catalytic domain of human 11-beta -hydroxysteroid dehydrogenase type 1. | Walker, EA., et al. 2001. J Biol Chem. 276: 21343-50. PMID: 11294832
- Expression and putative role of 11 beta-hydroxysteroid dehydrogenase isozymes within the human eye. | Rauz, S., et al. 2001. Invest Ophthalmol Vis Sci. 42: 2037-42. PMID: 11481269
- Improved lipid and lipoprotein profile, hepatic insulin sensitivity, and glucose tolerance in 11beta-hydroxysteroid dehydrogenase type 1 null mice. | Morton, NM., et al. 2001. J Biol Chem. 276: 41293-300. PMID: 11546766
- The human gene for 11 beta-hydroxysteroid dehydrogenase. Structure, tissue distribution, and chromosomal localization. | Tannin, GM., et al. 1991. J Biol Chem. 266: 16653-8. PMID: 1885595
- 11β-HSD1 contributes to age-related metabolic decline in male mice. | Morgan, SA., et al. 2022. J Endocrinol. 255: 117-129. PMID: 36205523
- Recycling glucocorticoids: therapeutic implications of the 11β-HSD1 enzyme system. | Tsai, KH. and Cooper, MS. 2023. J Endocrinol. 258: PMID: 37335193
- Targeting osteoblastic 11β-HSD1 to combat high-fat diet-induced bone loss and obesity. | Zhong, C., et al. 2024. Nat Commun. 15: 8588. PMID: 39362888
- Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme. | Albiston, AL., et al. 1994. Mol Cell Endocrinol. 105: R11-7. PMID: 7859916
- Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2. | Brown, RW., et al. 1996. Biochem J. 313 (Pt 3): 1007-17. PMID: 8611140
- 11 beta-hydroxysteroid dehydrogenase type II in human colon: a new marker of fetal development and differentiation in neoplasms. | Takahashi, K., et al. 1998. Anticancer Res. 18: 3381-8. PMID: 9858912
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
11β-HSD1 Antibody (D-5) | sc-518168 | 200 µg/ml | $322.00 | |||
11β-HSD1 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-550684 | 200 µg Ab; 40 µg BP | $361.00 | |||
11β-HSD1 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-550686 | 200 µg Ab; 20 µg BP | $361.00 | |||
11β-HSD1 Antibody (D-5) AC | sc-518168 AC | 500 µg/ml, 25% agarose | $424.00 | |||
11β-HSD1 Antibody (D-5) HRP | sc-518168 HRP | 200 µg/ml | $322.00 | |||
11β-HSD1 Antibody (D-5) FITC | sc-518168 FITC | 200 µg/ml | $336.00 | |||
11β-HSD1 Antibody (D-5) PE | sc-518168 PE | 200 µg/ml | $349.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 488 | sc-518168 AF488 | 200 µg/ml | $364.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 546 | sc-518168 AF546 | 200 µg/ml | $364.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 594 | sc-518168 AF594 | 200 µg/ml | $364.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 647 | sc-518168 AF647 | 200 µg/ml | $364.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 680 | sc-518168 AF680 | 200 µg/ml | $364.00 | |||
11β-HSD1 Antibody (D-5) Alexa Fluor® 790 | sc-518168 AF790 | 200 µg/ml | $364.00 |