Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γ-catenin Antibody (11E4): sc-59986. Western blot analysis of γ-catenin expression in non-transfected 293T: sc-117752 (A), mouse γ-catenin transfected 293T: sc-125099 (B) and HeLa (C) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γ-catenin CRISPR/Cas9 KO Plasmid (m): sc-421210
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γ-catenin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the γ-catenin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ-catenin Antibody (A-6): sc-514115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γ-catenin CRISPR/Cas9 KO Plasmid (m) | sc-421210 | 20 µg | $397.00 |