β-FR CRISPR/Cas9 KO Plasmid (h): sc-403346
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β-FR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the β-FR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β-FR Antibody (4B12): sc-293199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-FR CRISPR/Cas9 KO Plasmid (h) | sc-403346 | 20 µg | $397.00 |
Overview
FOLR2 encodes beta-folate receptor (β-FR), a glycosylphosphatidylinositol-anchored cell-surface receptor that binds folates and mediates their uptake, supporting one-carbon metabolism and nucleotide biosynthesis. Through regulation of intracellular folate availability, β-FR influences DNA synthesis and methylation-dependent processes that intersect with proliferative and differentiation programs. In humans, FOLR2 is frequently used as a marker of specific myeloid and macrophage subpopulations and is studied in contexts where folate-dependent metabolism and immune cell state are remodeled. Altered FOLR2 expression patterns are associated with inflammatory microenvironments and disease-relevant immune remodeling, motivating mechanistic studies in primary cells and model systems.
β-FR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOLR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOLR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOLR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish β-FR protein expression.
This CRISPR knockout system enables efficient generation of FOLR2-deficient cell models for investigation of β-FR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOLR2 exon(s) critical for β-FR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOLR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by β-FR CRISPR/Cas9 KO Plasmid (h) and β-FR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOLR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by β-FR HDR Plasmid (h) and β-FR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOLR2 homology arms to support homology-directed repair at defined FOLR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.