α-tectorin CRISPR/Cas9 KO Plasmid (h): sc-405876
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α-tectorin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α-tectorin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α-tectorin Antibody (2A5): sc-517128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-tectorin CRISPR/Cas9 KO Plasmid (h) | sc-405876 | 20 µg | $397.00 |
Overview
TECTA encodes α-tectorin, a major non-collagenous glycoprotein of the inner ear tectorial membrane that helps organize extracellular matrix architecture required for mechanoelectrical coupling in cochlear hair cells. α-tectorin contributes to membrane stiffness and micromechanics that support stereocilia deflection and frequency tuning, linking extracellular matrix assembly and proteostasis processes to auditory signal transduction. Variants in TECTA are associated with hereditary hearing loss phenotypes, making it a relevant target for studying genotype–structure–function relationships in cochlear extracellular matrices. Loss of α-tectorin function is commonly investigated in models of tectorial membrane development, hair cell biomechanics, and auditory pathway physiology.
α-tectorin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TECTA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TECTA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TECTA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α-tectorin protein expression.
This CRISPR knockout system enables efficient generation of TECTA-deficient cell models for investigation of α-tectorin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TECTA exon(s) critical for α-tectorin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TECTA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α-tectorin CRISPR/Cas9 KO Plasmid (h) and α-tectorin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TECTA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α-tectorin HDR Plasmid (h) and α-tectorin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TECTA homology arms to support homology-directed repair at defined TECTA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.