Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α-internexin CRISPR/Cas9 KO Plasmid (h): sc-402920
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α-internexin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α-internexin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α-internexin Antibody (G-9): sc-271302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-internexin CRISPR/Cas9 KO Plasmid (h) | sc-402920 | 20 µg | $397.00 |
Overview
INA encodes α-internexin, a neuronal intermediate filament protein that contributes to cytoskeletal architecture and axonal caliber by assembling into filament networks with other neurofilament subunits. It supports neurite outgrowth, axon stability, and intracellular transport by organizing the intermediate filament system within developing and mature neurons. Dysregulation of intermediate filament dynamics, including altered INA expression or filament organization, is frequently examined in the context of neuronal vulnerability, axonopathy, and protein-aggregation–associated neurodegenerative processes. As a neuron-enriched structural protein, α-internexin is widely used as a marker and mechanistic entry point for studies of neuronal differentiation and cytoskeletal remodeling.
α-internexin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the INA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the INA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the INA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α-internexin protein expression.
This CRISPR knockout system enables efficient generation of INA-deficient cell models for investigation of α-internexin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting INA exon(s) critical for α-internexin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple INA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α-internexin CRISPR/Cas9 KO Plasmid (h) and α-internexin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the INA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α-internexin HDR Plasmid (h) and α-internexin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by INA homology arms to support homology-directed repair at defined INA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.