RPA194 Antibody (F-6): sc-46699

RPA194 Antibody (F-6) is a mouse monoclonal IgG2b antibody that detects RPA194 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPA194 is the largest subunit of RNA polymerase I, playing a crucial role in the transcription of ribosomal RNA, which is essential for ribosome biogenesis and, consequently, protein synthesis in cells. Located primarily in the nucleolus, RPA194′s presence is vital for the assembly and stability of the RNA polymerase I complex, as RPA194 interacts with other subunits like RPA40 and RPA16 to form a functional transcription machinery. The integrity of RPA194 is critical, as RPA194 not only contributes to the transcription of class I genes but also ensures the proper functioning of RNA polymerase III, highlighting RPA194′s importance in cellular growth and proliferation. RPA194 (F-6) antibody is an invaluable tool for researchers studying ribosomal RNA synthesis and the regulatory mechanisms governing ribosome production, providing insights into fundamental biological processes and potential implications in diseases associated with ribosomal dysfunction.

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RPA194 Antibody (F-6) References:

1. Small molecule BMH-compounds that inhibit RNA polymerase I and cause nucleolar stress.  |  Peltonen, K., et al. 2014. Mol Cancer Ther. 13: 2537-46. PMID: 25277384
2. Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint.  |  Wei, T., et al. 2018. Cell Rep. 23: 404-414. PMID: 29642000
3. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer.  |  Morral, C., et al. 2020. Cell Stem Cell. 26: 845-861.e12. PMID: 32396863
4. Sempervirine inhibits RNA polymerase I transcription independently from p53 in tumor cells.  |  Caggiano, C., et al. 2020. Cell Death Discov. 6: 111. PMID: 33298840
5. Advances at the Interface of Cancer and Systemic Sclerosis.  |  Mecoli, CA., et al. 2021. J Scleroderma Relat Disord. 6: 50-57. PMID: 34124375
6. RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.  |  Moudry, P., et al. 2022. Cell Death Differ. 29: 687-696. PMID: 34611297
7. Discovery and Evaluation of Novel Angular Fused Pyridoquinazolinonecarboxamides as RNA Polymerase I Inhibitors.  |  Dorado, TE., et al. 2022. ACS Med Chem Lett. 13: 608-614. PMID: 35450366
8. POLR1A variants underlie phenotypic heterogeneity in craniofacial, neural, and cardiac anomalies.  |  Smallwood, K., et al. 2023. Am J Hum Genet. 110: 809-825. PMID: 37075751
9. Expression of RNA polymerase I catalytic core is influenced by RPA12.  |  Ford, BL., et al. 2023. PLoS One. 18: e0285660. PMID: 37167337
10. Nucleolar structure connects with global nuclear organization.  |  Wang, C., et al. 2023. Mol Biol Cell. 34: ar114. PMID: 37610836

The preferred RPA194 antibody is RPA194 Antibody (C-1): sc-48385.