Pax-6 Antibody (AD1.5): sc-53106

Pax-6 Antibody (AD1.5) is a mouse monoclonal IgG1 antibody that detects Pax-6 in mouse, rat, human, avian, and zebrafish by applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Pax-6 is a crucial transcriptional regulator that plays a significant role in eye development and is essential for the formation of various ocular structures. Anti-Pax-6 antibody (AD1.5) orchestrates the expression of genes necessary for eye development, and mutations in the Pax-6 gene can lead to severe congenital conditions such as aniridia, characterized by iris hypoplasia and potential blindness. Pax-6 is involved in other anterior segment malformations, including Peters anomaly, which can result in corneal clouding and other serious developmental issues. Pax-6 features a paired-type DNA-binding domain, composed of two distinct subdomains that interact with specific DNA sequences, allowing regulation of target genes effectively. The human Pax-6 gene produces two alternatively spliced isoforms, each with unique structural characteristics that contribute to regulatory functions. With broad species reactivity and proven applications, Pax-6 (AD1.5) monoclonal antibody serves as an invaluable tool for researchers studying eye development and related disorders.

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Pax-6 Antibody (AD1.5) References:

1. Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein.  |  Cvekl, A., et al. 1999. Invest Ophthalmol Vis Sci. 40: 1343-50. PMID: 10359315
2. Pax-6 expression and activity are induced in the reepithelializing cornea and control activity of the transcriptional promoter for matrix metalloproteinase gelatinase B.  |  Sivak, JM., et al. 2000. Dev Biol. 222: 41-54. PMID: 10885745
3. Pax-6 activates endogenous proglucagon gene expression in the rodent gastrointestinal epithelium.  |  Trinh, DK., et al. 2003. Diabetes. 52: 425-33. PMID: 12540617
4. Pax-6 and c-Maf functionally interact with the alpha-cell-specific DNA element G1 in vivo to promote glucagon gene expression.  |  Gosmain, Y., et al. 2007. J Biol Chem. 282: 35024-34. PMID: 17901057
5. Analysis of Non-Sumoylated and Sumoylated Isoforms of Pax-6, the Master Regulator for Eye and Brain Development in Ocular Cell Lines.  |  Liu, F., et al. 2018. Curr Mol Med. 18: 566-573. PMID: 30636604
6. Pax 6 Controls Neural Crest Potential of Limbal Niche Cells to Support Self-Renewal of Limbal Epithelial Stem Cells.  |  Chen, SY., et al. 2019. Sci Rep. 9: 9763. PMID: 31278274
7. DNA-binding and transactivation properties of Pax-6: three amino acids in the paired domain are responsible for the different sequence recognition of Pax-6 and BSAP (Pax-5).  |  Czerny, T. and Busslinger, M. 1995. Mol Cell Biol. 15: 2858-71. PMID: 7739566
8. Pax-QNR/Pax-6, a paired- and homeobox-containing protein, recognizes Ets binding sites and can alter the transactivating properties of Ets transcription factors.  |  Plaza, S., et al. 1994. Gene Expr. 4: 43-52. PMID: 7841787
9. Evolution of paired domains: isolation and sequencing of jellyfish and hydra Pax genes related to Pax-5 and Pax-6.  |  Sun, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 5156-61. PMID: 9144207
10. Pax-6, Prox 1, and Chx10 homeobox gene expression correlates with phenotypic fate of retinal precursor cells.  |  Belecky-Adams, T., et al. 1997. Invest Ophthalmol Vis Sci. 38: 1293-303. PMID: 9191592

The preferred Pax-6 antibody is Pax-6 Antibody (PAX6): sc-81649.