Date published: 2025-9-9

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Waf1/Cip1/CDKN1A p21 항체 (AC8.3): sc-53393

4.5(2)
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데이터 시트
  • Waf1/Cip1/CDKN1A p21 항체(AC8.3) 는 마우스 monoclonal IgG1κ, 4간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 히스타글루타닌-태그 재조합체 human p21 Waf1/Cip1에 대해 제기됨
  • WB, IP, IF 와IHC(P)으로 human origin의 p21 Waf1/Cip1을 검출할것을 권장합니다.
  • See Waf1/Cip1/CDKN1A p21 (F-5): sc-6246 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 Waf1/Cip1/CDKN1A p21 항체 (AC8.3) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Waf1/Cip1/CDKN1A p21 항체(AC8.3) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

더보기

이제 사이클린은 동족 사이클린 의존성 키나제 (Cdks)와 연관되고 활성화하는 능력을 통해 세포 주기 전환을 촉진하는 데 긍정적인 역할을 한다는 것이 잘 확립되어 있다. Cdk2는 사이클린 A, D 및 E와 연관되어 있으며 포유류의 G1에서 S로의 단계 전환 제어와 관련이 있다. p21 Waf1/Cip1, Cip1 또는 WAF1로 명명된 새로운 Cdk 상호 작용 단백질이 사이클린 A, 사이클린 D1, 사이클린 E 및 Cdk2 면역 침전물에서 확인되었다. p21 Waf1/Cip1은 Cdk의 강력하고 긴밀한 결합 억제제이며 사이클린 A-Cdk2, 사이클린 E-Cdk2, 사이클린 D1-Cdk4 및 사이클린 D2-Cdk4 복합체에 의한 Rb의 인산화를 억제할 수 있다. p21 Waf1/Cip1의 발현은 야생형에 의해 유도되지만 돌연변이에 의해 유도되지는 않는다. p21 Waf1/Cip1의 마우스 상동체는 CAP20으로 지정된다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Waf1/Cip1/CDKN1A p21 항체 (AC8.3)

sc-53393
200 µg/ml
$316.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgG Fc BP-HRP 번들

sc-536953
200 µg Ab; 10 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgGκ BP-HRP 번들

sc-534130
200 µg Ab; 40 µg BP
$354.00

Waf1/Cip1/CDKN1A p21 (AC8.3): m-IgG1 BP-HRP 번들

sc-544919
200 µg Ab; 20 µg BP
$354.00

Can p21 (AC8.3): sc-53393 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, p21 (AC8.3): sc-53393 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. This particular p21 antibody is not the best choice for IHC. Instead, p21 (F-5): sc-6246 is a directly conjugated mouse monoclonal antibody that can be easily used for IHC. It has many possible conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 5 out of 5 by from Good nuclear and cytoplasmic staining withGood nuclear and cytoplasmic staining with paraffin-embedded human breast tissue. -SCBT QC
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Waf1/Cip1/CDKN1A p21 항체 (AC8.3) is rated 4.5 out of 5 by 2.
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