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IL-6 항체 (10E5): sc-57315

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데이터 시트
  • IL-6 항체(10E5) 는 마우스 monoclonal IgG2bκ, 166간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • rat의 전체 길이 재조합 IL-6에 대해 제기됨
  • WB, IP, FCM와 ELISA으로 mouse와 rat origin의 IL-6을 검출할것을 권장합니다.
  • IL-6 (10E5): sc-57315무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP 는 IL-6 항체 (10E5) WB 애플리케이션용입니다. 이 시약은 이제 IL-6 항체 (10E5)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

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IL-6 항체 (10E5)는 WB, IP, FCM 및 ELISA에 의해 마우스 및 랫트 출처의 IL-6를 검출하는 IgG2b κ 마우스 단클론 IL-6 항체 (IL-6 항체 또는 인터류킨 6 항체로도 지정됨)이다. IL-6 항체 (10E5)는 비-접합 항-IL-6 항체 형태로 사용할 수 있다. 인터류킨-6 또는 IL-6는 숙주 방어, 면역 반응 및 조혈에 중요한 역할을 하는 212개의 아미노산 길이의 다기능 단백질이다. IL-6는 표피 랑게르한스 세포에 의해 구성적으로 발현되며, 그 발현은 자극된 각질 세포에서 유도된다. IL-6, IL-1β 및 TNFα는 내인성 피로겐으로 작용하여 세균 침입에 대한 발열 반응을 조절한다. IL-6 수용체는 IL-6 특이적 α 사슬과 IL-6, IL-11, CNTF, OSM 및 LIF 수용체에 공통적인 gp130 당단백질의 동종이량체로 구성된 삼량체 복합체이다. IL-6를 자극하면 gp130이량체로 형성되고 관련 키나제 JAK1 및 JAK2가 활성화된다. 일단 활성화되면 JAK1 및 JAK2는 Stat3를 인산화하여 Stat3 반응성 유전자의 핵 전위 및 전사를 유발한다. IL-6는 또한 NFIL6 전사를 조절하는 Ras/MAP 키나아제 경로를 활성화하는 것으로 나타났다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

IL-6 항체 (10E5)

sc-57315
100 µg/ml
$316.00

IL-6 (10E5): m-IgG Fc BP-HRP 번들

sc-539053
100 µg Ab; 10 µg BP
$354.00

IL-6 (10E5): m-IgG2b BP-HRP 번들

sc-549728
100 µg Ab; 10 µg BP
$354.00

How can I diluete for IL-6 product with BSA or milk powder? Which do you suggest dilution rate For WB?

Asked by: ayse
Thank you for your question. We recommend using UltraCruz® Blocking Reagent: sc-516214, which is the reagent we employ when testing our antibodies.
Answered by: Tech Support Europe
Date published: 2025-01-28

how i purchase il-6 antibody in India from Jaipur (Rajasthan)

Asked by: kavita2021
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-26

Can we use this IL-6 (10E5) antibody (sc-57315) for IHC?

Asked by: Abhi
Thank you for your question. IL-6 Antibody (10E5): sc-57315 is only recommended for detection of IL-6 of mouse and rat origin by WB, IP, FCM and ELISA. We do not recommend it for use in IHC or IHC(P) applications.
Answered by: Tech Support Europe
Date published: 2022-07-14

What is the molecular weight of IL-6 ?

Asked by: AbPolly
Thank you for your question. IL-6 has an expected molecular weight of approximately 21 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from WB on rat hippocampusThe sc-57315 antibody was tested (1: 200,ON 4°) on samples extracted from rat hippocampus using a 12% acrylamide gel. In figure (from left to right): 30ug, 60ug, 80ug and 100ug of extract. The signal was slightly non-specific, but a more intense band was observed around 21kDa, as suggested by the datasheet. [Marker (from the bottom): 11,17,22,25,32 kDa]
Date published: 2022-06-23
Rated 3 out of 5 by from It doesn't work in WB of secretome of C2C12We have used this antibody to detect Il6 on conditioned medium of C2C12 cells at 1:1000 diluition and it doesn't work. It could be that the amount of IL6 produced by these cells is too low
Date published: 2018-10-05
Rated 5 out of 5 by from Works well in rat frozen liver sectionsRat liver frozen sections were subjected to immunofluorescence using IL-6 (10E5) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Positive signals in portal tracts were clear without any antigen retrieval.
Date published: 2018-03-14
Rated 5 out of 5 by from Work very wellHave been using this antibody for Western blot detecting IL-6 in mouse tissue and C2C12 samples. Always got clear, specific band. would like to recommend it.
Date published: 2018-03-11
Rated 4 out of 5 by from Good!I got a small sample and tried it for WB in a dilution of 1:300. The results were great.
Date published: 2018-03-02
Rated 5 out of 5 by from Skeletal muscle immunostainingBright staining of nuclei in frozen skeletal muscle samples.
Date published: 2017-11-22
Rated 4 out of 5 by from Good antibodyformalin fixed rat brain , IF staining, 1:100 dilution. got good result
Date published: 2017-10-24
Rated 3 out of 5 by from 总体效果一般该抗体不能与人体组织反应,是一个重要的缺陷。在使用过程中,在动物组织中检测该蛋白的表达改变,需要适当增加上样量,提高1抗的浓度。
Date published: 2017-09-28
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IL-6 항체 (10E5) is rated 4.2 out of 5 by 27.
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