안티-Id4 항체 (B-5)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 Id4 를 감지하는 데에 추천한다.; 이외에 porcine등 species와 반응할수 있습니다
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Id4 항체 (B-5)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-365656 X, 200 µg/0.1 ml)이 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Id4 Antibody (B-5) for WB applications. These reagents are now offered in bundles with Id4 Antibody (B-5) (see ordering information below).
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Id4 Antibody (B-5) is a high quality monoclonal Id4 antibody (also designated ID4 antibody, or DNA-binding protein inhibitor ID-4 antibody) suitable for the detection of the Id4 protein of mouse, rat and human origin. Id4 Antibody (B-5) is available as both the non-conjugated anti-Id4 antibody form, as well as multiple conjugated forms of anti-Id4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 (1-3), Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins (1-5). Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can Id4 (B-5): sc-365656 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Id4 (B-5): sc-365656 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 5 out of
Xiaobing He from
Id4 antibody (B-5) works great for IF stainingI tried the Id4 antibody (B-5) on both 293T cells and human Id4 transfected 293T cells for the immunofluorescence staining at 1:300. It gave very specific nuclear staining on the endogenous Id4 in non-transfected 293T cells. For the human Id4 transfected 293T cells, the Id4 shows both cytoplasmic and nuclear staining pattern, which is consistent with the published data reporting Id4 cytoplasmic and nuclear localization in prostate cancer cells.
Date published: 2018-04-19
Rated 3 out of
Rat samples without bandI'm sorry that the sample is only submitting the report until now. It's a busy time. Taking the time to try this antibody, found good effect in 293T cells: closed fast closed closed 30min beyotime liquid, first antibody is the product (1:700 dilution), second antibogy buy from abclonal anti mouse (1:30000 dilution), the exposure time is 60 seconds.The molecular weight is about 18KD.But there was no band on the rat BMSC cells, and that was the case for the two time. It is also necessary to continue to work hard.The picture is the result of 293T cell samples
Date published: 2018-01-04
Rated 1 out of
only background, no bands at expected size in WBIn a large panel of cell lines expressing high levels of ID4 we didn't get a specific signal at the expected protein size of 18kDa, however the overall background was quite strong. We used the antibody in TBST, 5% BSA diluted 1:100.
Date published: 2017-11-18
Rated 2 out of
Joshua Chen from
No good for WBAntibody specificity is not good. The main band is not at the expected site (~15Kd）.
Date published: 2017-09-11
Rated 3 out of
Works in Embryonic Neural Tissue for IHCWorks fine in embryonic neural tissue 1:200 O/N with antigen retrieval, a little dirty.
Date published: 2017-08-17
Rated 1 out of
it is not good at IHCwe tested this antibody in mouse, pig, dog, cow tissue sample.
ID4 expression in the tissues was shown in other papers using other antibody and transgenic animal.
but, by this antibody, not detect the ID4.
So i want refund
Date published: 2017-02-17
Rated 5 out of
Produced positive Western blot data of Id4Produced positive Western blot data of Id4 expression in non-transfected and human Id4 transfected 293T whole cell lysates. -SCBT QC