Date published: 2025-9-7

001 800-1338-3838

SCBT Portrait Logo
Seach Input

HSPA5/BiP/GRP78 항체 (76-E6): sc-13539

4.4(25)
리뷰 쓰기질문하기

데이터 시트
  • HSPA5/BiP/GRP78 항체 76-E6 는 랫드 monoclonal IgG HSPA5/BiP/GRP78 항체, 104간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • mouse의 전체 길이 재조합 GRP 78에 대해 제기됨
  • HSPA5/BiP/GRP78 항체 (76-E6)는 WB and IP으로 mouse, rat and human유래의 GRP 78 를 감지하는 데에 추천한다.
  • 항-HSPA5/BiP/GRP78 항체(76-E6)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • HSPA5/BiP/GRP78 (76-E6): sc-13539무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 HSPA5/BiP/GRP78 항체(76-E6)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

빠른 링크

더보기

GRP 78 항체 (76-E6)는 WB와 IP에 의해 마우스, 랫 및 인간 출처의 GRP 78 단백질을 검출하는 IgG 랫 단클론 GRP 78 항체(GRP78 항체, HSPA5 항체 또는 BIP 항체로도 지정됨)이다. GRP 78 항체 (76-E6)는 비 conjug 결합 항-GRP 78 항체 형태뿐만 아니라 아가로오스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 다중 결합 형태의 항-GRP 78 항체로 사용할 수 있다. HSP 70 계열은 4가지 고도로 보존된 단백질인 HSP 70, HSC 70, GRP 75 및 GRP 78로 구성된다. 이 단백질은 다양한 역할을 한다. 다중 protein 복합체의 조립을 용이하게 하는 분자 샤페론으로 작용하고 세포막과 핵을 가로질러 폴리펩티드의 전위에 참여하며 초기 폴리펩티드 사슬의 적절한 접힘을 돕는다. HSP 70을 제외한 계열의 모든 구성체는 영장류 세포에서 구성적으로 발현된다. HSP 70 발현은 열 스트레스에 대한 반응으로 강하게 유도된다. HSP 70과 HSC 70은 세포질 내질 망상체 및 미토콘드리아 수입 기계에서 중요한 역할을 하며 포유류 세포의 세포질과 핵 모두에서 발견된다. HSP 70과 HSC 70은 모두 초기 폴리펩티드 사슬의 샤페론화와 부적절하게 접힌 단백질의 축적으로부터 세포를 보호하는 데 관여한다. GRP 78은 소포체에 국한되어 수입 분비 단백질을 받고 초기 펩티드 사슬의 접힘 및 전위에 관여한다. GRP 75 발현은 미토콘드리아 매트릭스로 제한되며 핵과 미토콘드리아 출처의 초기 폴리펩티드 사슬의 전위 및 접힘을 돕는다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

HSPA5/BiP/GRP78 항체 (76-E6) 참고문헌:

  1. 샤페론인 Hsp60에 의한 열 스트레스 하에서의 단백질 변성 방지.  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
  2. 낮은 배양 온도가 마우스 FM3A 세포에서 hsp70 mRNA의 유도 및 hsp70 및 hsp105의 축적에 미치는 영향.  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
  3. 정상 및 허혈성 뇌에서 발현되는 쥐 grp75, Hsp70 가족 구성원 및 그 발현의 복제.  |  Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
  4. 진핵생물에서 단백질 매개 단백질 성숙.  |  Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
  5. Hsp70 단백질이 힘을 생성하는 모터로 작용할 수 있을까요?  |  Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
  6. 인간 미토콘드리아 hsp70의 복제 및 세포 내 국소화.  |  Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
  7. grp94(엔도플라즈민)의 자가 인산화.  |  Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776

주문정보

제품명카탈로그 번호 단위가격수량관심품목

HSPA5/BiP/GRP78 항체 (76-E6)

sc-13539
200 µg/ml
$316.00

HSPA5/BiP/GRP78 항체 (76-E6) AC

sc-13539 AC
500 µg/ml, 25% agarose
$416.00

HSPA5/BiP/GRP78 항체 (76-E6) HRP

sc-13539 HRP
200 µg/ml
$316.00

HSPA5/BiP/GRP78 항체 (76-E6) FITC

sc-13539 FITC
200 µg/ml
$330.00

HSPA5/BiP/GRP78 항체 (76-E6) PE

sc-13539 PE
200 µg/ml
$343.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 488

sc-13539 AF488
200 µg/ml
$357.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 546

sc-13539 AF546
200 µg/ml
$357.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 594

sc-13539 AF594
200 µg/ml
$357.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 647

sc-13539 AF647
200 µg/ml
$357.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 680

sc-13539 AF680
200 µg/ml
$357.00

HSPA5/BiP/GRP78 항체 (76-E6) Alexa Fluor® 790

sc-13539 AF790
200 µg/ml
$357.00

I see the western blot using this antibody gives a double band pattern at expected molecular weight. Are both the bands representing GRP78 and why is that? If only one of them is GRP78, which one is it?

Asked by: Phei000
Thank you for your question. Please contact technical service by emailing scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-08-13

Hello, Is the HSPA5/BiP/GRP78 Antibody (76-E6) sc-13539 raised against SBD of GRP78 (can you tell the sequence)? Is the epitope known for this antibody? Thank you!

Asked by: Ausra
Thank you for your question. HSPA5/BiP/GRP78 Antibody (76-E6): sc-13539 was raised against full length GRP 78 of mouse origin. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2023-10-10

Is this GRP 78(76-E6): sc-13539 antibody can use for IF?  I found it has been tested in some paper.

Asked by: wending
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-05-27

Hello. Is this antibody suitable for evaluation of expression of cell surface GRP78 and cytoplasmic GRP78? Thank you in advance!

Asked by: rrebelo
Thank you for your question. This antibody is not currently recommended for IF or IHC(P). We do not currently have any antigen localization data using this antibody. There are a few product citations that we are aware of using this antibody for IF or IHC. We recommend you review those references for any relevant information. For example, PMID's: 32184660, 30221722 and 31767864.
Answered by: Technical Support
Date published: 2020-07-09

Fiz um wester blot e apareceu duas bandas bem próximas em torno de 75 e 78 porque isso acontece?

Asked by: FRANCIELE
We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to your local distributor in Brazil or contact Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: Tech Service
Date published: 2020-02-21

请问这个抗体的二抗是抗大鼠的二抗吗?

Asked by: yang200214yy
Thank you for your inquiry.GRP 78 Antibody (76-E6) is a rat monoclonal IgG.So you should use conjugated anti-rat secondary antibody. 感谢您的提问。GRP 78 (76-E6)是大鼠来源的单克隆抗体。您应该使用抗大鼠的二抗。
Answered by: Technical Support 9
Date published: 2019-11-27

Dear sir, I would like to apply for this antibody-(76-E6): sc-13539,but I don't know how to get it,would you please give me a help for applying for this? thank you

Asked by: Qinzi
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, please contact Customer Service in China.
Answered by: Tech Service
Date published: 2019-05-07

I would like to use your GRP78 Ab conjugated with Alexa 647 for FACS analysis. I would like to know which Isotype do you recommend for your control. And do you have data showing the expression of GRP78 by FACS with your AB?

Asked by: DeborahM
Thank you for your inquiry and your interest in this product. As isotype control we would suggest to use our sc-516643 normal rat IgG Alexa Fluor® 647. The GRP 78 Antibody (76-E6): sc-13539 is recommended for detection of GRP 78 of mouse, rat and human origin by WB and IP. We have not yet tested this antibody in flow cytometry. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Support Europe
Date published: 2017-12-29
  • y_2025, m_8, d_27, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_ko_KR, sid_13539, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 113ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug of protein sample. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 1 out of 5 by from Concern about antibody specilificyWe used this antibody on a clean membrane and received a single band. However, this band is not at the expected size of GRP78 but significantly lower. We received this result in two cell lines with endogenous expression of GRP78 (HEK 293 and HCT-116).
Date published: 2024-06-27
Rated 1 out of 5 by from The blot comes out below the molecular weightDetection by wb in HEK-293 resulted in a band below 70 kDa
Date published: 2024-02-21
Rated 5 out of 5 by from Good result!I used this Ab (1:500, 16 h 4 °C) to blot the GRP78 in SH-SY5Y cells (30 ug total lysate), secondary antibody was mouse (1:1000, 1 h room temp). The band was very clear and strong at 70-75 kDa.
Date published: 2023-03-23
Rated 5 out of 5 by from 条带清晰本次的实验条带还是很清晰的,已经发表了一篇文章,下次还会继续支持购买。许愿有个好的实验结果!!!!!
Date published: 2022-11-01
Rated 3 out of 5 by from Be aware of the target!This antibody also targets HSP70! So please interpret your data carefully!!
Date published: 2020-08-23
Rated 5 out of 5 by from WB resultWe used this Ab (1:800) to blot the BIP in HepG2 cells. The band was very clear and strong.
Date published: 2020-08-22
Rated 5 out of 5 by from Ottimo anticorpoTestato su lisato piastrinico. Segnale davvero bello e pulito! visibile facilmente con 50 ug di lisato. Da fare attenzione solo al MW, leggermente più basso di 78 KDa.
Date published: 2020-07-30
  • y_2025, m_8, d_27, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_25
  • loc_ko_KR, sid_13539, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
HSPA5/BiP/GRP78 항체 (76-E6) is rated 4.4 out of 5 by 25.
  • y_2025, m_8, d_27, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_25
  • loc_ko_KR, sid_13539, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 113ms
  • REVIEWS, PRODUCT