Date published: 2025-9-9

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Estrogen Receptor alpha 항체 (D-12): sc-8005

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데이터 시트
  • Estrogen Receptor alpha 항체(D-12) 는 마우스 monoclonal IgG2aκ, 290간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 아미노 산2-185 mapping at the N-terminus of Estrogen Receptor alpha of human 의하고 기원한다
  • 안티-Estrogen Receptor alpha 항체 (D-12)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Estrogen Receptor alpha 를 감지하는 데에 추천한다.
  • 항-Estrogen Receptor alpha 항체(D-12)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • IF, IHC(P) 와 FCM에 사용가능한 TRITC 결합제품이 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-8005 X, 200 µg/0.1 ml)이 있습니다.
  • Estrogen Receptor alpha (D-12): sc-8005무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 는 Estrogen Receptor alpha 항체(D-12) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Estrogen Receptor alpha 항체(D-12) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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에스트로겐 수용체 알파 항체(D-12)는 인간 에스트로겐 수용체 알파(ERα)의 N-말단에 있는 2-185번 아미노산에 대해 생성된 마우스 단일 클론 IgG2a 카파 경쇄 항체입니다. ERα 단일클론항체(D-12)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 섹션의 면역조직화학(IHCP), 효소결합 면역흡착 분석법(ELISA)을 통해 쥐, 쥐, 인간에서 유래된 ERα를 검출합니다. ERα 단백질은 주로 에스트로겐 반응 세포의 핵에 위치하며, 이 곳에서 ERα는 스테로이드/갑상선 호르몬 수용체 슈퍼패밀리에 속하는 리간드 활성화 전사 인자로서 기능합니다. ERα의 핵 내 국소화는 매우 중요합니다. 왜냐하면 ERα는 에스트로겐 반응 요소(EREs)에서 DNA와 직접 상호 작용을 가능하게 함으로써 세포 증식, 분화, 생식 기능에 관여하는 유전자의 전사를 조절하기 때문입니다. 이러한 국소화는 정상적인 성적 발달과 생식 건강을 포함한 에스트로겐의 생리적 효과를 매개하는 데 필수적입니다. ERα의 국소화 오류 또는 조절 오류는 유전자 발현의 변화를 초래할 수 있으며, 유방암과 같은 호르몬 의존성 암과 같은 다양한 질병과 관련이 있습니다. ERα 단일 클론 항체(D-12)는 비접합형과 다중 접합형 형태로 제공되며, HRP, FITC, 아가로스, PE, 다양한 Alexa Fluor® 접합체를 포함하고 있어, ERα의 기능, 국소화, 질병에서의 역할에 대한 광범위한 연구 응용 분야에 다양하게 활용할 수 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Estrogen Receptor alpha 항체 (D-12)

sc-8005
200 µg/ml
$316.00

Estrogen Receptor alpha (D-12): m-IgG Fc BP-HRP 번들

sc-525445
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha (D-12): m-IgG2a BP-HRP 번들

sc-546259
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha 항체 (D-12) X

sc-8005 X
200 µg/0.1 ml
$316.00

Estrogen Receptor alpha 항체 (D-12) AC

sc-8005 AC
500µg/1ml, 25% agarose
$416.00

Estrogen Receptor alpha 항체 (D-12) HRP

sc-8005 HRP
200 µg/ml
$316.00

Estrogen Receptor alpha 항체 (D-12) FITC

sc-8005 FITC
200 µg/ml
$330.00

Estrogen Receptor alpha 항체 (D-12) PE

sc-8005 PE
200 µg/ml
$343.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 488

sc-8005 AF488
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 546

sc-8005 AF546
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 594

sc-8005 AF594
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 647

sc-8005 AF647
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 680

sc-8005 AF680
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) Alexa Fluor® 790

sc-8005 AF790
200 µg/ml
$357.00

Estrogen Receptor alpha 항체 (D-12) TRITC

sc-8005 TRITC
200 µg/ml
$320.00

I would like to know if this antibody recognize the whole sequence from 2- 185, or just part of the sequence within?

Asked by: Anonymous
Thank you for your question. Estrogen Receptor alpha Antibody (D-12): sc-8005 was raised against amino acids 2-185 mapping at the N-terminus of Estrogen Receptor alpha of human origin. Its binding site is one specific point within this epitope region.
Answered by: Tech Support Europe
Date published: 2020-07-02

Hi, I want to do IHC for formalin fixed rat mammary gland, does this antibody work for rat tissues by IHC?

Asked by: Yanrong
Thank you for your question. Yes, this antibody is recommended for IHC(P) in mouse, rat and human tissues.
Answered by: Technical Support
Date published: 2019-08-17

Can we use this antibody for IF and IHC staining in paraffin-embedded mouse tissues?

Asked by: YuHsin
Thank you for your question. ERα Antibody (D-12): sc-8005 is recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so using it for IF or IHC(P) staining should work well. For published data with this antibody in murine tissues and IHC(P) please see PMID 19332863 or PMID 22992387.
Answered by: Tech Support Europe
Date published: 2019-07-03

Can we use this antibody in rat brain samples for W.B. Immunoflourescence in frozen sections?

Asked by: Rukmani p
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, is suitable for Western blotting (WB) and immunoflourescence (IF) in frozen tissues of rat origin. We have not tested this antibody specifically in rat brain tissue samples. Please refer to the data on our website for our recommended positive controls to include when testing this antibody in your samples.
Answered by: Technical Support
Date published: 2017-08-03

Can I use this antibody for immunoprecipitation of ER alpha from mouse brain?

Asked by: Victor86
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, can be used for immunoprecipitation (IP) in mouse samples. We are not aware of any specific publications citing use of the antibody to IP ER alpha from brain tissue samples, but it has been successfully used for IP in both human and mouse cell lines.
Answered by: Technical Support
Date published: 2017-05-18

I need to use an antibody to detect the protein in samples of canineorigin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-21

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to Fluorescein (sc-8005 FITC) and TRITC (sc-8005 TRITC). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 5 out of 5 by from Great IHC results on mouse brain tiisueAntigen retrieval with TE buffer works really great!
Date published: 2022-07-31
Rated 5 out of 5 by from Great ProductAn excellent product for the lab's research. Highly recommended for future uses.
Date published: 2022-07-13
Rated 3 out of 5 by from Ok, it workedWe tried the free sample on wester blot at the suggested dilution 1:200 on mouse samples. It worked, but compared to the old policlonal MC-20the result isn't good as before.
Date published: 2021-12-21
Rated 4 out of 5 by from ChIPWorking well so far for my current ChIP experiments.
Date published: 2020-12-28
Rated 3 out of 5 by from good antibodygood antibody for western blotting and also immunofluorescence
Date published: 2019-01-21
Rated 2 out of 5 by from Unsure of molecular weight in Western blotA very faint band showed up at the correct molecular weight. A brighter band showed up at the incorrect molecular weight. Western Blot (1:100) in rat white adipose tissue.
Date published: 2018-09-12
Rated 4 out of 5 by from Good antibodyI tried it on cultured human cells on IF (1:100, 1:200), both concentration worked.
Date published: 2018-04-07
Rated 5 out of 5 by from GreatI had great signal for ICC on Human breast cancer cell lines.
Date published: 2018-02-27
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Estrogen Receptor alpha 항체 (D-12) is rated 3.9 out of 5 by 24.
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