ERK 1/2 항체 (MK1) WB, IP와 IF으로 mouse, rat, human와 canine origin의 ERK 1 and ERK 2을 검출할것을 권장합니다.

ERK 1/2 항체 (MK1): sc-135900

ERK 1/2 항체 (MK1) is rated 4.4 out of 5 by 14.
  • y_2020, m_10, d_24, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_ko_KR, sid_135900, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 150ms
  • REVIEWS, PRODUCT
5
9
4
3
3
1
2
0
1
1

Datasheets

    • ERK 1/2 항체(MK1) 는 마우스 monoclonal mouse IgG1 κ(kappa light chain) 이며 50 µg/0.5 ml으로 제공합니다.
    • rat origin의 ERK 1에서 내부에 위치한 325-345 아미노산을 항원으로 사용하였습니다.
    • WB, IP와 IF으로 mouse, rat, human와 canine origin의 ERK 1 and ERK 2을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • See ERK 2 (D-2): sc-1647 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • ERK 1/2 (MK1): sc-135900무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anonymous
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-22

Will this ERK1/2 antibody detect the protein in bovine samples?

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2016-12-22

Can ERK2 (SC-135900) be used as a loading control for western blot lysates?

Asked by: Eldhose
  • y_2020, m_10, d_24, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_ko_KR, sid_135900, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 174ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent antibody for WBs I have used this antibody at 1:1000 dilution for western blotting using a number of human cell lines and obtained a very strong signal. Extremely pleased with this antibody.
Date published: 2019-06-15
Rated 1 out of 5 by from No signal in In-Cell Western This antibody did not work for my In-Cell Western assays in HEK293. I use 4% paraformaldehyde fixation and methanol permeabilization, and I don't know if it could work with a different protocol. I would not purchase this antibody for any immunocytochemistry approaches.
Date published: 2018-05-07
Rated 5 out of 5 by from good result This antibody can be used to publish using the antibody in WB and IHC in mouse neuron.
Date published: 2017-05-28
Rated 5 out of 5 by from good ERK 1/2 antibody worked well in the rat cerebrum tissue for WB analysis
Date published: 2017-05-13
Rated 5 out of 5 by from Works very well It was used for WB on rat cerebrum tissue extract., which shows good quality and got bands very clearly.
Date published: 2017-04-24
Rated 5 out of 5 by from positive band Dilution detection 1:1000. It shows the positive band, I am satisfied with the result.
Date published: 2017-02-26
Rated 3 out of 5 by from Low sensitivity for WB This ab has good specificity, but relatively low sensitivity for WB with ERK 1/2 from human, mouse and rat.
Date published: 2017-01-30
Rated 4 out of 5 by from band is clear Western work well in human protein samples.Do not detect the antibody in IHC of IF
Date published: 2016-12-29
  • y_2020, m_10, d_24, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_ko_KR, sid_135900, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT

산타크루즈바이오테크놀러지는 생물의학연구에 필요한 제품을 개발하는 선구자입니다. Toll Free 1-800-457-3801로 연락주세요. Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. 판권소유. "Santa Cruz Biotechnology", 와 Santa Cruz Biotechnology, Inc. 로고, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", 및 San Juan Ranch 로고, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz"와 "EZ Touch" 는 Santa Cruz Biotechnology, Inc의 등록된 상표입니다. 모든 상표는 각자 소유주의 소유입니다.