Date published: 2025-9-7

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COL1A2 항체 (E-6): sc-393573

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데이터 시트
  • COL1A2 항체(E-6) 는 마우스 monoclonal IgG1 λ COL1A2 항체, 19간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 mouse origin의 Collagen α2 Type I의 내부에 위치한 1064-1093의 아미노산에 해당됩니다.
  • COL1A2 항체 (E-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Collagen α2 Type I 를 감지하는 데에 추천한다.
  • 항-COL1A2 항체(E-6)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • COL1A2 (E-6): sc-393573무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)가 COL1A2 항체(E-6) 가 WB and IHC(P)에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다. 이 시약은COL1A2 抗体 (E-6) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)。기타m-IgGλ BP 커플링제는 작은 쥐 IgG 결합 단백질완정한 리스트를 참고 해 주시길 바랍니다.

빠른 링크

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COL1A2 항체(E-6)는 마우스, 쥐 및 인간 유래의 COL1A2 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 λ 마우스 모노클로날 COL1A2 항체(콜라겐 제1형 알파 2 사슬(COL1A2) 항체라고도 함)입니다. COL1A2 항체(E-6)는 비접합 항-COL1A2 항체 형태와 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-COL1A2 항체 모두로 제공됩니다. 광범위한 COL 유전자 제품군(콜라겐)은 피브릴 형성 간질성 콜라겐(유형 I, II, III, V)과 기저막 콜라겐(유형 IV)을 포함한 여러 사슬 유형으로 구성되며 각 유형에는 여러 동형체가 포함되어 있습니다. 콜라겐은 인장 강도가 높은 섬유질 세포 외 기질 단백질로 힘줄과 연골과 같은 결합 조직의 주요 구성 요소입니다. 모든 콜라겐은 삼중 나선 도메인을 포함하고 있으며 복잡한 결합 조직을 형성하기 위해 종종 측면 자기 결합을 보입니다. 또한 여러 콜라겐은 정상적인 조직 구조와 기능을 유지하는 데 중요한 세포 접착에 중요한 역할을 합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

COL1A2 항체 (E-6)

sc-393573
200 µg/ml
$316.00

COL1A2 (E-6): m-IgGλ BP-HRP 번들

sc-524005
200 µg Ab, 40 µg BP
$354.00

COL1A2 항체 (E-6) AC

sc-393573 AC
500 µg/ml, 25% agarose
$416.00

COL1A2 항체 (E-6) HRP

sc-393573 HRP
200 µg/ml
$316.00

COL1A2 항체 (E-6) FITC

sc-393573 FITC
200 µg/ml
$330.00

COL1A2 항체 (E-6) PE

sc-393573 PE
200 µg/ml
$343.00

COL1A2 항체 (E-6) Alexa Fluor® 488

sc-393573 AF488
200 µg/ml
$357.00

COL1A2 항체 (E-6) Alexa Fluor® 546

sc-393573 AF546
200 µg/ml
$357.00

COL1A2 항체 (E-6) Alexa Fluor® 594

sc-393573 AF594
200 µg/ml
$357.00

COL1A2 항체 (E-6) Alexa Fluor® 647

sc-393573 AF647
200 µg/ml
$357.00

COL1A2 항체 (E-6) Alexa Fluor® 680

sc-393573 AF680
200 µg/ml
$357.00

COL1A2 항체 (E-6) Alexa Fluor® 790

sc-393573 AF790
200 µg/ml
$357.00

COL1A2 (E-6) 중화펩타이드

sc-393573 P
100 µg/0.5 ml
$68.00

您好,我之前问的问题不是能不能透膜,您回答的不是我的问题,我问的问题是拿荧光标记的这个抗体做流式的时候,是否需要透膜?这个抗体结合的部位是细胞内还是细胞外?谢谢。Do the cells need to be permeabilized for the antibody to bind?

Asked by: dxl3742
感谢提问。 目前我们所掌握的信息,正常情况下这个蛋白定位是细胞外,谢谢。 Thank you for your question. The protein is located extracellular.
Answered by: Ling Chen
Date published: 2022-07-11

Hello, for flow cytometry is this stained directly or do the cells need to be permeabilized?

Asked by: dxl3742
Thank you for your question. If the experiment requires us to do cell permeability, we will do. 感谢提问。 如果实验需要,我们可以进行细胞通透。
Answered by: Ling Chen
Date published: 2022-07-07

マウスの組織で、WBとIFあるいはIHCを考えています。 CollagenⅠ A1との交差反応が見られるのでしょうか。 あるいはA1は染まらず、A2のみ特異的に検出できるのでしょうか。

Asked by: Tom0112
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at asia@scbio.cn, or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メールasia@scbio.cnまたはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

To my question about IHC", you mentioned the product COL1A2 (E-6): sc-393573. But the description of the product suggest "HRP (sc-393573 HRP)" for IHC(P). Is there no difference between the products "sc-393573" and "sc-393573 HRP" for IHC(P) and WB?

Asked by: David27
Thank you for your question. The difference between the catalog numbers, sc-393573 and sc-393573 HRP, is that the latter is the primary antibody directly conjugated to horse radish peroxidase (HRP). This antibody is suitable for WB and IHC(P), and if you purchase the unconjugated antibody, you will need to use a secondary antibody, or mouse IgG binding protein for detection. Alternatively, if you prefer to use a directly conjugated primary antibody, you can purchase the HRP conjugated antibody and also use it for both WB and IHC(P).
Answered by: Technical Support
Date published: 2023-09-23

Also, what is the recommended secondary antibody in IHC? There are only those written for IF, WB etc

Asked by: David27
Thank you for your question. We would recommend using our HRP-conjugated mouse IgG binding protein, m-IgGκ BP-HRP (sc-516102), for indirect immunoperoxidase staining, in lieu of conventional anti-mouse secondary antibodies. Alternatively, we would recommend if you are using the Avidin-Biotin Complex (ABC) staining protocol to select any conventional biotinylated anti-mouse IgG secondary antibody and use in conjunction with our ImmunoCruz® ABC Kit (sc-516216).
Answered by: Technical Support
Date published: 2017-07-20

What is the recommended agent for antigen unmasking in IHC

Asked by: David27
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval methods using this antibody, COL1A2 (E-6): sc-393573. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-20

What application is the blocking peptide sc-393573 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 15
Date published: 2023-10-21
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Rated 5 out of 5 by from This antibody works very well for IFC and IHQThis antibody works very well for immunofluorescence and immunhistochemistry (mouse lung tissue)
Date published: 2023-06-20
Rated 3 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:25
Date published: 2018-11-19
Rated 4 out of 5 by from COL1A2 Antibody (E-6)COL1A2 (E-6): sc. Near-infrared western blot analysis of LDH-A expression in MEF whole cell lysates. Blocked with 5% NFDM for 1 hour. Detection reagent used: DAM CW800 (Licor).
Date published: 2018-06-28
Rated 5 out of 5 by from 抗体很不错抗体很好用,特异性好,就是效价低了点,其他都不错,Santa的抗体就是很好用,不错的选择,还送优惠券
Date published: 2018-04-28
Rated 5 out of 5 by from great antibodyIt is great for western blot and staining for mouse heart sample.
Date published: 2017-12-14
Rated 4 out of 5 by from Good antibody for IFI Used 1:200 dilution in paraffined mouse kidney. I have got good IF photos.
Date published: 2017-08-28
Rated 4 out of 5 by from good for WBI use 1:500 dilution and get clear band at 130kd, but have some undesired bands
Date published: 2017-08-18
Rated 5 out of 5 by from The antibody is goodThis antibody is very sutiable for WB, it shows a very clear band.
Date published: 2017-07-25
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COL1A2 항체 (E-6) is rated 4.6 out of 5 by 16.
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