CD3 항체 (5B2) IP, IF와 FCM으로 mouse, rat와 human origin의 CD3을 검출할것을 권장합니다.

CD3 항체 (5B2): sc-70626

CD3 항체 (5B2) is rated 5.0 out of 5 by 2.
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데이터 시트

    • CD3 항체 5B2 는 마우스 monoclonal IgG3 이며 100 µg/ml으로 제공합니다.
    • raised against human peripheral lymphocytes
    • IP, IF와 FCM으로 mouse, rat와 human origin의 CD3을 검출할것을 권장합니다.
    • IF, IHC(P) 와 FCM에 사용가능한 FITC결합제품이 있습니다.
    • See CD3 (PC3/188A): sc-20047 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Should I permeabilize the cells if I want use the 5B2 for FACS analysis?

Asked by: Elenarb000
Thank you for your question. Yes, we recommend fixing and permeabilizing when using this mouse monoclonal antibody, CD3 (5B2): sc-70626, for flow cytometry (FCM) for FACS applications. A link to our recommended protocol for FCM is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-09-20

I am using CD3 (5B2): sc-70626 monoclonal antibody for immunoprecipitation, how much antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from IF publication with rat C6 cells IF publication with rat C6 cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from CD3 (5B2) was used successfully in mouse D1 and D1 CD3 (5B2) was used successfully in mouse D1 and D1-CD4 T cells for immunoprecipitation. -SCBT Publication Review
Date published: 2013-04-25
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