ATR Antibody (C-1): sc-515173

ATR Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ATR antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATR, also known as ataxia-telangiectasia and Rad3-related protein, plays a crucial role in the cellular response to DNA damage, particularly in the activation of cell cycle checkpoints. ATR phosphorylates key proteins involved in the DNA damage response, such as checkpoint kinase CHK1 and tumor suppressor BRCA1, making ATR vital for maintaining genomic stability. ATR is primarily located in the nucleus, where ATR forms intranuclear foci in response to DNA damage or replication stress, highlighting ATR′s importance in cellular repair mechanisms. ATR is essential for early embryonic development, underscoring ATR′s significance in both normal cellular function and the prevention of diseases associated with genomic instability.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATR Antibody (C-1) References:

1. Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4.  |  Schmidt, DR. and Schreiber, SL. 1999. Biochemistry. 38: 14711-7. PMID: 10545197
2. ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK.  |  Hall-Jackson, CA., et al. 1999. Oncogene. 18: 6707-13. PMID: 10597277
3. An overactivated ATR/CHK1 pathway is responsible for the prolonged G2 accumulation in irradiated AT cells.  |  Wang, X., et al. 2003. J Biol Chem. 278: 30869-74. PMID: 12791699
4. MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation.  |  Wang, Y. and Qin, J. 2003. Proc Natl Acad Sci U S A. 100: 15387-92. PMID: 14657349
5. A novel protein activity mediates DNA binding of an ATR-ATRIP complex.  |  Bomgarden, RD., et al. 2004. J Biol Chem. 279: 13346-53. PMID: 14724280
6. The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.  |  Jones, GG., et al. 2004. Oncogene. 23: 1911-21. PMID: 14755251
7. Inhibition of ATR leads to increased sensitivity to hypoxia/reoxygenation.  |  Hammond, EM., et al. 2004. Cancer Res. 64: 6556-62. PMID: 15374968
8. ATR takes a crack at the nuclear envelope.  |  Smolka, MB. and Lammerding, J. 2023. Mol Cell. 83: 3588-3590. PMID: 37863026
9. FBXW7-loss Sensitizes Cells to ATR Inhibition Through Induced Mitotic Catastrophe.  |  O'Brien, S., et al. 2023. Cancer Res Commun. 3: 2596-2607. PMID: 38032106
10. First-in-Human Study of the Ataxia Telangiectasia and Rad3-Related (ATR) Inhibitor Tuvusertib (M1774) as Monotherapy in Patients with Solid Tumors.  |  Yap, TA., et al. 2024. Clin Cancer Res. 30: 2057-2067. PMID: 38407317
11. cDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein.  |  Cimprich, KA., et al. 1996. Proc Natl Acad Sci U S A. 93: 2850-5. PMID: 8610130
12. The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.  |  Keegan, KS., et al. 1996. Genes Dev. 10: 2423-37. PMID: 8843195

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATR Antibody (C-1) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-ATR Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATR Antibody (C-1) Specifications

Alternate Product Name: ATR

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2596-2621

Epitope Target: ATR

Epitope Species: human

Product Reactivity/Detection: ATR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for ATR Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin