Date published: 2025-9-8

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NUAK1/ARK5 항체 (B-4): sc-271828

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데이터 시트
  • NUAK1/ARK5 항체(B-4) 는 마우스 monoclonal IgG2aκ, 1간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 NUAK1을 항원으로 사용하였습니다.
  • NUAK1/ARK5 항체(B-4)는 WB, IP, IF and ELISA으로 NUAK1 를 감지하는 데에 추천한다 .
  • 항-NUAK1/ARK5 항체(B-4)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • NUAK1/ARK5 (B-4): sc-271828무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP 는 NUAK1/ARK5 항체 (B-4) WB 애플리케이션용입니다. 이 시약은 이제 NUAK1/ARK5 항체 (B-4)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

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연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

NUAK1/ARK5 항체 (B-4) 참고문헌:

  1. NUAK1 녹다운은 마이크로RNA-30b-5p를 통해 전립선암 세포의 상피-중간엽 전이, 이동 및 침입을 억제합니다.  |  Guan, Y., et al. 2018. Int J Clin Exp Pathol. 11: 5694-5704. PMID: 31949655
  2. KIF20A/NUAK1/Nrf2/GPX4 신호 경로를 억제하면 페로셉토시스가 유도되고 대장암의 옥살리플라틴에 대한 민감도가 향상됩니다.  |  Yang, C., et al. 2021. Aging (Albany NY). 13: 13515-13534. PMID: 33819186
  3. DKK1은 간세포암종에서 Akt 활성화를 통해 NUAK1 전사 발현을 촉진합니다.  |  Yao, CY., et al. 2023. Cell Biol Int. 47: 383-393. PMID: 36480792
  4. NUAK1은 MYPT1/PP1β 및 GSK3β 의존적 PLK4 조절을 통해 췌장암에서 중심체 복제를 조절합니다.  |  Whyte, D., et al. 2023. Mol Oncol. 17: 1212-1227. PMID: 36975767
  5. NUAK1은 식도 편평상피세포암에서 슬러그 전사를 상향 조절하여 종양 전이를 촉진합니다.  |  Yang, H., et al. 2023. Cancer Cell Int. 23: 258. PMID: 37919754
  6. NUAK1은 성장 인자에 의존하는 mTORC2 및 Akt 신호의 활성화를 조정합니다.  |  Palma, M., et al. 2023. Cell Biosci. 13: 232. PMID: 38135881
  7. AMPK 관련 키나아제 NUAK1은 미토콘드리아 대사 기능을 국소적으로 조절하여 대뇌 피질 축삭 분지를 제어합니다.  |  Lanfranchi, M., et al. 2024. Nat Commun. 15: 2487. PMID: 38514619
  8. NUAK1은 카스파제 6에 의한 발열과 염증을 활성화하여 대사 기능 장애와 관련된 지방간염 진행을 촉진합니다.  |  Sheng, M., et al. 2024. Hepatol Commun. 8: PMID: 38967580
  9. NUAK1은 STAT5/GLI1/SOX2 신호를 활성화하여 암세포 확장을 촉진하고 위암의 화학 내성을 유도합니다.  |  Cao, L., et al. 2024. Cell Rep. 43: 114446. PMID: 38996065
  10. 비소세포폐암에서 오시머티닙 내성을 촉진하기 위해 NADK의 NUAK1-매개 인산화가 ROS 축적을 완화합니다.  |  Lin, W., et al. 2024. Cancer Res. 84: 4081-4098. PMID: 39159134

주문정보

제품명카탈로그 번호 단위가격수량관심품목

NUAK1/ARK5 항체 (B-4)

sc-271828
200 µg/ml
$316.00

NUAK1/ARK5 (B-4): m-IgG Fc BP-HRP 번들

sc-525828
200 µg Ab; 10 µg BP
$354.00

NUAK1/ARK5 (B-4): m-IgG2a BP-HRP 번들

sc-546432
200 µg Ab; 10 µg BP
$354.00

NUAK1/ARK5 항체 (B-4) AC

sc-271828 AC
500 µg/ml, 25% agarose
$416.00

NUAK1/ARK5 항체 (B-4) HRP

sc-271828 HRP
200 µg/ml
$316.00

NUAK1/ARK5 항체 (B-4) FITC

sc-271828 FITC
200 µg/ml
$330.00

NUAK1/ARK5 항체 (B-4) PE

sc-271828 PE
200 µg/ml
$343.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 488

sc-271828 AF488
200 µg/ml
$357.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 546

sc-271828 AF546
200 µg/ml
$357.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 594

sc-271828 AF594
200 µg/ml
$357.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 647

sc-271828 AF647
200 µg/ml
$357.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 680

sc-271828 AF680
200 µg/ml
$357.00

NUAK1/ARK5 항체 (B-4) Alexa Fluor® 790

sc-271828 AF790
200 µg/ml
$357.00

Do you have any idea, why the protein runs at 55-60 kDa instead of 75 kDa? Other antibodies here by SC detect the protein at 80-90 kDa. Thank you for you ideas on that!

Asked by: nchatain
Thank you for your question. Observed molecular weight can vary due to post-translational processing and alternative splicing of the protein. While splicing isoforms have only been described based on computational data, there are a number of post-translational modifications such as sumoylation and phosphorylation that may contribute to the protein's molecular weight in western blots.
Answered by: Tech Support Europe
Date published: 2025-05-23

Have you guys tested if this antibody cross reacts with mouse ARK5?

Asked by: TKim
Thank you for your question, Yes this antibody is mouse, rat, and human reactive. -Tech Service
Answered by: Tech Service 1
Date published: 2021-02-04

Hello. I was curious if you could shed some light on why this antibody picks up a 55 kDa piece of ARK5 when it is listed as 74 kDa. Is there a particular processing event that results in this fragment? Thank you for your help!

Asked by: Anna13
Thank you for your question. The Western blot data which you are referring to on our website appears to be inconsistent with the expected observed molecular weight for ARK5 of ~ 74 kDa, as well as with Western blot data using different antibodies against ARK5. As a result, our labs are now reviewing this data and the data will likely be removed and replaced.
Answered by: Technical Support
Date published: 2017-10-06

What is the recommended fixation method for immunofluorescence staining with ARK5 (B-4): sc-271828 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from mehrere Proteinbanden bei erwarteten kDa-Bereichim Hirngewebe (Kortex-Lysat) von wildtyp oder transgenen Mäusen mehrere Proteinbanden im erwarteten Molekulargewichtsbereich.
Date published: 2018-01-31
Rated 5 out of 5 by from Great immunofluorescence nuclear and cytoplasmicGreat immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Nice Western Blot data of ARK5 expression in SaosNice Western Blot data of ARK5 expression in Saos-2 and A549 whole cell lysates. -SCBT QC
Date published: 2014-09-14
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NUAK1/ARK5 항체 (B-4) is rated 4.7 out of 5 by 3.
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