Vimentin Antibody (V9): sc-6260

Vimentin Antibody (V9) is a mouse monoclonal IgG1 kappa light chain antibody raised against purified vimentin from porcine eye lens, a critical cytoskeletal protein primarily expressed in mesenchymal cells. Vimentin mouse monoclonal antibody (V9) recognizes an epitope within the vimentin immunogen sequence and demonstrates broad species cross-reactivity, including mouse, rat, and human samples, in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and flow cytometry (FCM) applications. Anti-vimentin monoclonal antibody (V9) is available in non-conjugated form and multiple conjugated forms including horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, agarose, and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. Vimentin belongs to the intermediate filament family and maintains cellular structure, integrity, and mechanical resilience. Vimentin interacts with numerous proteins including cytokeratins, actin, and microtubules to form an intricate network of cytoskeletal fibers. Vimentin′s association with motor proteins facilitates intracellular transport and organelle positioning. While predominantly cytoplasmic, vimentin localizes to the nucleus and cell membrane under specific conditions. Vimentin serves as a key marker for epithelial-to-mesenchymal transition (EMT), a process crucial in wound healing, development, and tumor metastasis. Vimentin′s expression pattern becomes particularly significant in cancer biology, as vimentin frequently appears upregulated in various neoplasms including melanoma, sarcomas, and breast carcinomas, where vimentin often shows co-expression with cytokeratins and other intermediate filaments. Vimentin (V9) antibody enables researchers to investigate cytoskeletal dynamics, cancer progression, and tissue-specific expression patterns through vimentin′s consistent cytoplasmic and membrane staining properties.

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Vimentin Antibody (V9) References:

1. A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.  |  Dráberová, E., et al. 1986. Folia Biol (Praha). 32: 295-303. PMID: 2465190
2. Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord.  |  Lukás, Z., et al. 1989. Histochem J. 21: 693-701. PMID: 2482269
3. Vimentin regulates activation of the NLRP3 inflammasome.  |  dos Santos, G., et al. 2015. Nat Commun. 6: 6574. PMID: 25762200
4. Vimentin Diversity in Health and Disease.  |  Danielsson, F., et al. 2018. Cells. 7: PMID: 30248895
5. Coexpression of intermediate filament polypeptides in human fetal and adult tissues.  |  Van Muijen, GN., et al. 1987. Lab Invest. 57: 359-69. PMID: 3669613
6. Extracellular vimentin as a versatile immune suppressive protein in cancer.  |  van Loon, K., et al. 2023. Biochim Biophys Acta Rev Cancer. 1878: 188985. PMID: 37717859
7. Hard alpha-keratin IF: a structural model lacking a head-to-tail molecular overlap but having hybrid features characteristic of both epidermal keratin and vimentin IF.  |  Parry, DA. 1995. Proteins. 22: 267-72. PMID: 7479699
8. What can be learned from intermediate filament gene regulation in the mouse embryo.  |  Duprey, P. and Paulin, D. 1995. Int J Dev Biol. 39: 443-57. PMID: 7577435
9. Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord.  |  Lukás, Z., et al. 1993. Histochemistry. 100: 495-502. PMID: 8163392
10. Intermediate filament structure and assembly.  |  Stewart, M. 1993. Curr Opin Cell Biol. 5: 3-11. PMID: 8448027
11. Species-specificity of glial vimentin as revealed by immunocytochemical studies with the Vim 3B4 and V9 monoclonal antibodies.  |  Gereben, B., et al. 1995. Neurobiology (Bp). 3: 151-64. PMID: 8563714
12. Structural and biological consequences of increased vimentin expression in simple epithelial cell types.  |  Andreoli, JM. and Trevor, KT. 1995. Cell Motil Cytoskeleton. 32: 10-25. PMID: 8674130

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vimentin Antibody (V9) has 1,158 citations in a variety of scientific publications.

Primary antibodies like Anti-Vimentin Antibody (V9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vimentin Antibody (V9) Specifications

Alternate Product Name: Vimentin

Product Clone Name: V9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Vimentin

Epitope Species: porcine

Product Reactivity/Detection: Vimentin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,158 citations in various scientific publications

Available Conjugates/Forms for Vimentin Antibody (V9): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine