VCAM-1 Antibody (P3C4): sc-20070

VCAM-1 Antibody (P3C4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Vascular Cell Adhesion Molecule 1 (VCAM-1) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). VCAM-1 is a transmembrane sialoglycoprotein predominantly expressed on activated endothelial cells, playing a pivotal role in mediating the adhesion of leukocytes to the endothelium during immune responses. This adhesion is crucial for the recruitment of immune cells to sites of inflammation, which is particularly significant in the context of inflammatory diseases such as atherosclerosis and rheumatoid arthritis. The structure of VCAM-1 comprises seven immunoglobulin-like domains, which are essential for high binding affinity to integrins like α4β1 and α4β7 found on leukocytes. These structural features not only facilitate leukocyte adhesion but also participate in signal transduction pathways that regulate cell survival and proliferation, underscoring VCAM-1′s importance in maintaining vascular integrity and immune function. VCAM-1 (P3C4) antibody serves as an invaluable tool for researchers aiming to elucidate the mechanisms of endothelial activation and vascular inflammation, enabling exploration of VCAM-1′s interactions with integrins and other proteins involved in immune cell trafficking and endothelial dysfunction.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VCAM-1 Antibody (P3C4) References:

1. Vascular cell adhesion molecule-1 augments adenovirus-mediated gene transfer.  |  Chu, Y., et al. 2001. Arterioscler Thromb Vasc Biol. 21: 238-42. PMID: 11156859
2. Expression of vascular cell adhesion molecule-1 in human renal allografts.  |  Briscoe, DM., et al. 1992. J Am Soc Nephrol. 3: 1180-5. PMID: 1282831
3. Serum soluble vascular cell adhesion molecule-1 (VCAM-1) concentrations in children with reflux nephropathy.  |  Kaneyama, K., et al. 2005. Pediatr Surg Int. 21: 39-42. PMID: 15372288
4. Cytokine stimulated vascular cell adhesion molecule-1 (VCAM-1) ectodomain release is regulated by TIMP-3.  |  Singh, RJ., et al. 2005. Cardiovasc Res. 67: 39-49. PMID: 15949468
5. MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1.  |  Harris, TA., et al. 2008. Proc Natl Acad Sci U S A. 105: 1516-21. PMID: 18227515
6. Targeting Rapamycin to Podocytes Using a Vascular Cell Adhesion Molecule-1 (VCAM-1)-Harnessed SAINT-Based Lipid Carrier System.  |  Visweswaran, GR., et al. 2015. PLoS One. 10: e0138870. PMID: 26407295
7. 2-Methoxyestradiol prevents monocyte adhesion to vascular endothelial cells via downregulation of VCAM-1 expression.  |  Zhang, Y., et al. 2016. Gynecol Endocrinol. 32: 571-6. PMID: 26880304
8. Analysis of the Increase of Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression and the Effect of Exposure in a Hyperbaric Chamber on VCAM-1 in Human Blood Serum: A Cross-Sectional Study.  |  Van Damme-Ostapowicz, K., et al. 2022. Medicina (Kaunas). 58: PMID: 35056403
9. Comparison of vascular cell adhesion molecule 1 (VCAM-1) during pregnancy, after placental detachment and during puerperium between normal and pregnancy with COVID-19.  |  Pasaribu, HP., et al. 2023. Narra J. 3: e413. PMID: 38450333
10. Soluble vascular cell adhesion molecule-1 in perennial allergic rhinitis.  |  Ohashi, Y., et al. 1998. Acta Otolaryngol. 118: 105-9. PMID: 9504173

The preferred VCAM-1 antibody is VCAM-1 Antibody (E-10): sc-13160.